羊源<em>GADD45A</em>基因过表达Vero细胞系的构建及其对PPRV复制的影响

doi:10.3969/j.issn.1674-7968.2025.09.018

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Abstract Peste des petits ruminants virus (PPRV) infection induces diverse pathological damage in infected hosts and poses a serious threat to ruminant health. Transcriptome sequencing analysis of host cells following PPRV infection, conducted by our research group in the early stage, revealed a significant alteration in the expression level of the growth arrest and DNA damage-inducible 45A (GADD45A) gene. To investigate the potential role of GADD45A in PPRV replication, a recombinant lentiviral vector encoding the GADD45A gene was constructed, and a stable Vero cell line constitutively expressing GADD45A was established. qPCR, Western blot, and serial passage experiments confirmed sustained GADD45A expression at both mRNA and protein levels and demonstrated the stability of the cell line over multiple passages. The result showed that the established cell line maintained good viability and stable GADD45A expression throughout continuous passaging. The Vero cell line stably expressing GADD45A protein significantly enhanced the replication of PPRV. This study provides certain of biological tools and technical supports for further elucidating the molecular mechanisms underlying PPRV pathogenesis.

Key words： Growth arrest and DNA damage protein 45A gene (GADD45A) Lentiviral vector Cells line Peste des petits ruminants virus (PPRV) Sheep

Received: 23 January 2025

ZTFLH:

S855.3

Corresponding Authors: ^*yuan2918@126.com; shangyoujun@caas.cn

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	Articles by authors
	DING Hai-Yan
	YUAN Li-Gang
	ZHAO Jing-Han
	ZHENG Hai-Xue
	SHANG You-Jun
	LI Dan

Cite this article:

DING Hai-Yan,YUAN Li-Gang,ZHAO Jing-Han, et al. Establishment of a Sheep-derived Vero Cell Line Overexpressing GADD45A and Its Effect on PPRV Replication[J]. 农业生物技术学报, 2025, 33(9): 2082-2091.

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https://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2025.09.018 OR https://journal05.magtech.org.cn/Jwk_ny/EN/Y2025/V33/I9/2082

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