|
|
|
| Identification and Diversity Analysis of HMW-GS Composition and 1BL/1RS in Some New Wheat (Triticum aestivum) Varieties (Lines) in Henan Province |
| GENG Hui-Min1,*, WANG Meng-Na1, ZHANG Da-Le2 |
| School of Life Science, Luoyang Normal University, Luoyang 471934, China |
|
|
|
|
Abstract The genetic improvement of wheat (Triticum aestivum) processing quality is a crucial objective in breeding programs, while the composition of high molecular weight glutenin subunits (HMW-GS) and the presence of the 1BL/1RS translocation are 2 significant genetic factors that affecting the processing quality of wheat. In this study SDS-PAGE and acid-polyacrylamide gel electrophoresis (A-PAGE) were employed to systematically characterize the HMW-GS composition and 1BL/1RS translocation background in 151 new wheat varieties (lines) from the 2023 Henan provincial wheat regional trials. The results revealed 12 distinct HMW-GS subunit types, with 3 variants at the Glu-A1 locus, 5 at Glu-B1, and 4 at Glu-D1. The frequency of the high-quality subunit 5+10 was relatively low, accounting for only 19.21%. A total of 22 types of HMW-GS subunit combination forms were detected. The average quality score was 6.83 points. The subunit combination with the highest distribution frequency was 1/7+9/2+12 (accounting for 27.81%), while the high-quality subunit combination 1/7+8/5+10 only accounted for 8.61%. Additionally, 62 wheat varieties (lines) were identified as carrying the 1BL/1RS translocation chromosome, accounting for 41.06% of the tested materials. 14 wheat varieties (lines) have been identified, which possess HMW-GS with a score of 10 and were non-1BL/1RS translocation lines. These varieties (lines) hold potential value for breeding strong-gluten wheat. Therefore, the proportion of new wheat varieties (lines) containing high-quality HMW-GS combinations was not high, and the average subunit score was relatively low. However, the distribution frequency of the 1BL/1RS translocation lines had decreased. This study provides crucial genetic background information for wheat breeders in Henan province. The selected non-1BL/1RS translocation lines carrying high-quality subunit combinations could serve as important resources for developing high-yield and high-quality wheat varieties, offering significant guiding value for improving wheat processing quality.
|
|
Received: 24 April 2025
|
|
|
|
Corresponding Authors:
* Corresponding author, lyghm114@126.com
|
|
|
|
[1] 安悦. 2023. 湖北省小麦的HMW-GS组成及其与品质性状的相关分析[D]. 硕士学位论文, 华中农业大学, 导师: 任喜峰, pp. 54.
(An Y.2003. HMW-GS composition and its correlation with quality characters of wheat in Hubei Province[D]. Thesis for M.S, Huazhong Agriculture University, Suppervisor: Ren X F, pp. 54.)
[2] 陈加江, 逄孝云, 赵法茂, 等. 2009. 1BL/1RS易位系小麦谷蛋白和醇溶蛋白亚基组成、含量及其对加工品质性状的影响[J]. 中国粮油学报, 24(5): 11-15.
(Chen J J, Pang X Y, Zhao F M, et al.2009. Comparative investigations of gliadin and gluten in 1BL/1RS translocation lines[J]. Journal of the Chinese Cereals and Oils Association, 24(5): 11-15.)
[3] 范家霖, 陈晓杰, 张建伟, 等. 2021. 高分子量麦谷蛋白亚基组成及其与小麦品质性状的关系分析[J]. 麦类作物学报, 41(5): 544-552.
(Fan J L, Chen X J, Zhang J W, et al.2021. Composition of high molecular weight glutenin subunits and theirs relationship with wheat quality traits[J]. Journal of Triticeae Crops, 41(5): 544-552.)
[4] 范玉顶, 李斯深, 孙海艳, 等. 2005. HMW-GS与北方手工馒头加工品质关系的研究[J]. 作物学报, 31(1): 97-101.
(Fan Y D, Li S S, Sun H Y, et al.2005. Relationship between HMW-GS and making quality of northern style hand-made steamed bread[J]. Acta Agronomica Sinica, 31(1): 97-101.)
[5] 高晓慧, 任翠翠, 宋杰, 等. 2018. 黄淮南片麦区区试小麦品种(系)的遗传多样性及Pm21和1BL/1RS分子检测[J]. 西北农业学报, 27(6): 779-785.
(Gao X H, Ren C C, Song J, et al.2018. Diversity and detection of 1BL/1RS and Pm21 in wheat cultivars (lines) from regional trail in southern of Yellow and Huai River facultative winter wheat region[J]. Acta Agriculturae Boreali-occidentalis Sinica, 27(6):7 79-785.)
[6] 耿惠敏. 2007. 夏繁用于构建小麦遗传群体的可行性及1BL/1RS易位系影响高分子量谷蛋白亚基遗传的研究[D]. 博士学位论文, 四川农业大学, 导师: 任正隆,pp. 44-45.
(Geng H M.2007. Study on the feasibility of constructing genetic population using summer planting and the effect of 1BL/1RS translocation on the inheritance of HMW-GS[D]. Thesis for Ph.D., Sichuan Agricultural University, Suppervisor: Ren Z L, pp. 44-45.)
[7] 耿惠敏. 2023. 714个黄淮麦区小麦新品种(系)HMW-GS组成的研究[J]. 农业科技通讯,(10): 62-65+171.
(Geng H M.2023. The study on HMW-GS composition of 714 wheat cultivars (lines) from Huang-huai wheat area[J]. Bulletin of Agricultural Science and Technology,(10):62-65+171.)
[8] 李立, 龚梦婕, 李锁平, 等. 2018. 黄淮麦区小麦新品系高分子量谷蛋白亚基的组成及结构变化分析[J]. 华北农学报, 33(2): 157-162.
(Li L, Gong M J, Li S P, et al.Composition and structural changes of High-Molecular-Weight glutenin subunits in new wheat lines in the Huang-Huai wheat aera[J]. Acta Agriculturae Boreali-sinica, 33(2): 157-162.)
[9] 刘春雷, 杨雪, 张丽琴, 等. 2015. 722份河南省小麦新品系高分子量谷蛋白亚基多样性分析[J]. 河南农业科学, 44(4): 46-48.
(Liu C L, Yang X, Zhang L Q, et al.2015.Polymorphism analysis of HMW-GS in new wheat lines from Henan province[J]. Journal of Henan Agricultural Sciences, 44(4): 46-48.)
[10] 路辉丽, 彭星星, 尹豪, 等. 2024. 河南省小麦生产现状及优质小麦发展对策研究[J]. 粮油食品科技, 32(1): 185-192.
(Lu H L, Peng X X, Yin H,et al.2024. Research on the current situation of wheat production and development strategies for high quality wheat in Henan province[J]. Science and Technology of Cereals, Oils and Foods, 32(1): 185-192.)
[11] 马小乐, 李葆春, 孟亚雄, 等. 2015. 甘肃小麦品种1BL/1RS易位系鉴定及面筋熟制特性分析[J]. 农业生物技术学报, 23(06): 747-754.
(Ma X L, Li B C, Meng Y X, et al.2015. Identification of 1BL/1RS translocation lines and analysis of gluten cooking features of wheat (Triticum aestivum) germplasms in Gansu province[J]. Journal of Agricultural Biotechnology, 23(06): 747-754.)
[12] 孙允超. 2013. 小麦育种进程与现代育种方法分析[J]. 农业科技通讯, (6): 6-10.
(Sun Y C. 2013. Aanlysis of wheat breeding progress and modern breeding methods[J]. Bulletin of Agricultural Science and Technology, (6): 6-10.)
[13] 唐建卫, 刘建军, 张平平, 等. 2008. 贮藏蛋白组分对小麦面团流变学特性和食品加工品质的影响[J]. 中国农业科学, 41(10): 2937-2946.
(Tang J W, Liu J J, Zhang P P, et al.2008. Effects of gluten protein fractions on dough property and products quality in common wheat[J]. Scientia Agricultura Sinica, 41(10): 2937-2946.)
[14] 魏育明, 郑有良, 周荣华, 等. 2001. 几种鉴定小麦背景中1BL/1RS易位染色体的分子标记方法比较研究[J]. 四川农业大学学报, 19(1): 10-13.
(Wei Y M, Zheng Y L, Zhou R H, et al.2001. Comparison of molecular methods for identifying the presence of 1BL/1RS translocation chromosomes in wheat[J]. Journal of Sichuan Agricultural University, 19(1): 10-13.)
[15] 谢科军, 朱保磊, 孙家柱, 等. 2016. 黄淮南片小麦高分子量谷蛋白亚基组成及其与品质的关系[J]. 麦类作物学报, 36(5): 595-602.
(Xie K J, Zhu B L, Sun J Z, et al.2016. Composition of high molecular weight glutenin subunits of wheat varieties (lines) in Huanghuai Southern Region and their relationship with quality[J]. Journal of Triticeae Crops, 36(5): 595-602.)
[16] 晏本菊. 2004. 中国西南麦区普通小麦(Triticum aestivum L.)优质育种的生化遗传基础研究[D]. 博士学位论文, 四川农业大学, 导师: 任正隆, pp. 32-33.
(Yan B J.2004. Studiy on biochemical and genetic basis of quality breeding in common wheat (Triticum aestivum L.) in Southwest China[D]. Thesis for Ph.D., Sichuan Agricultural University, Suppervisor: Ren Z L, pp. 32-33.)
[17] 张丽琴, 刘春雷, 杨雪, 等. 2012. 黄淮麦区小麦新品种(系)高分子量谷蛋白亚基多态性分析[J]. 麦类作物学报, 32(11): 79-82.
(Zhang L Q, Liu C L, Y X, et al.2012. Polymorphism analysis of HMW-GS in new wheat varieties in Huang-huai wheat region of China[J]. Journal of Triticeae Crops, 32(11): 79-82.)
[18] 张维瑞, 张莉, 张大乐, 等. 2009. 黄淮麦区部分小麦新品种(系)醇溶蛋白遗传多样性分析[J]. 河南大学学报(自然科学版), 39(1): 63-67+80.
(Zhang W R, Zhang L, Zhang D L, et al.2002. Genetic diversity of gliadin in some new wheat varieties (lines) in Huanghuai wheat zone[J]. Journal of Henan University (Natural Science), 39(1): 63-67+80.)
[19] 张学勇, 庞斌双, 游光霞, 等. 2002. 中国小麦品种资源Glu-1位点组成概况及遗传多样性分析[J]. 中国农业科学, 35(11): 1302-1310.
(Zhang X Y, Pang B S, You G X, et al.2002. Allelic variation and genetic diversity at Glu-1 loci in Chinese wheat (Triticum aestivum L.) germplasms[J]. Scientia Agricultura Sinica, 35(11): 1302-1310.
[20] 周阳, 何中虎, 张改生, 等. 2004. 1BL/1RS易位系在我国小麦育种中的应用[J]. 作物学报, 30(6): 531-535.
(Zhou Y, He Z H, Zhang G S, et al.2004. Utilization of 1BL/1RS translocation in wheat breeding in China[J]. Acta Agronomica Sinica, 30(6): 531-535.)
[21] He Z H, Liu L, Xia X C, et al.2005. Composition of HMW and LMW glutenin subunits and their effects on dough properties, pan bread, and noodle quality of chinese bread wheats[J]. Cereal Chemistry, 82(4): 345-350.
[22] Liu J, Luo D, Li X, et al.2016. Effects of inulin on the structure and emulsifying properties of protein components in dough[J]. Food Chemistry, 210(11): 235-247.
[23] Ma M, Li Y, Xue C, et al.2021. Current situation and key parameters for improving wheat quality in China[J]. Frontiers in Plant Science, 12: 638525.
[24] Masci S, Dovidio R, Lafiandra D, et al.1998. Characterization of a low-molecular-weight glutenin subunit gene from bread wheat and the corresponding protein that represents a major subunit of the glutenin polymer[J]. Plant Physiology, 118(4): 1147-1158.
[25] Peter I, Payne Kathyrn G, Corfield, et al.1981. Correlations between the inheritance of certain high-molecular weight subunits of glutenin and bread-making quality in progenies of six crosses of bread wheat[J]. Journal of the Science of Food and Agriculture, 32: 51-60
[26] Payne P I, Lawrence G J.1983. Catalogue of alleles for complex gene loci, Glu-A1, Glu-B1 and Glu-D1 which code for high molecular-weight subunits of glutenin in hexaploid wheat[J]. Cereal Research Communications, 11:29-35
[27] Payne P I, Nightingale M A, Krattiger A F, Holt L M.1987b.The relationship between HMW glutenin subunit composition and the bread-making quality of British-grow wheat varieties[J]. Journal of the Science of Food & Agriculture, 40: 51-65 |
|
|
|
