独脚金内酯对棉纤维发育的影响及D27基因的表达特性分析

doi:10.3969/j.issn.1674-7968.2025.08.002

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Abstract As a plant hormone, strigolactones has a regulatory effect on cotton fiber development. This study utilized cotton (Gossypium) ovule culture technology to optimize the ovule culture system. 15 μmol/L strigolactone (GR24) and 0.1, 1, and 5 μmol/L Tis108 were added to treat cotton ovules to screen for Tis108 concentrations that inhibit fiber elongation and explore the effects of strigolactone and its inhibitors on ovule quality and fiber length; Using qRT-PCR to analyze the expression of the β-carotene isomerase D27 gene (D27) in cotton fibers on 5, 10, 15, 20, 25, and 30 d, which was involved in the synthesis pathway of lactone. The results showed that: 1) In 30 d cotton fibers, compared with the control group, treatment with 15 μmol/L GR24 increased cotton fiber length by 2.07 mm, and treatment with 5 μmol/L Tis108 decreased cotton fiber length by 2.22 mm; 2) Compared with the control group, the 6 Gossypium hirsutum D27 gene members showed higher expression levels and differential expression in fibers treated with GR24 on 5 d at different stages; 3) Fibers from 6 periods (5, 10, 15, 20, 25, and 30 d) in the island cotton field environment, 6 GbD27 genes showed different expression characteristics among the fibers. The expression of GbD27-4 gene was more significant in the fibers on 5 and 10 d. This study preliminarily explored the in vitro culture conditions of cotton ovules, screened suitable concentrations of strigolactones inhibitors, and found that the D27 gene was significantly expressed in the early stage of fiber development, provides a theoretical basis for further research on the molecular biology function of cotton D27 gene.

Key words： Cotton fiber β-carotene isomerase Ovule culture Expression analysis Strigolactone

Received: 21 November 2024

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Corresponding Authors: *zhengkai555@126.com

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	ZHOU Qiang
	LI Ying
	SUN Guo-Qing
	CHEN Qin
	CAI Yong-Sheng
	CHEN Quan-Jia
	QU Yan-Ying
	ZHENG Kai

Cite this article:

ZHOU Qiang,LI Ying,SUN Guo-Qing, et al. Effect of Strigolactones on Cotton Fiber Development and Analysis of Expression Characteristics of D27 Gene[J]. 农业生物技术学报, 2025, 33(8): 1661-1673.

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https://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2025.08.002 OR https://journal05.magtech.org.cn/Jwk_ny/EN/Y2025/V33/I8/1661

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