月季<em>RhMYB23</em>基因的克隆及功能分析

doi:10.3969/j.issn.1674-7968.2025.08.003

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Abstract The MYB transcription factors constitute one of the largest families of transcription factors in plants and are extensively involved in plant growth and development as well as the biosynthesis of various secondary metabolites. To explore the regulatory function of MYB transcription factors in the biosynthesis of proanthocyanidins in Rosa hybrida, this study used R. hybrida 'Violet' as the experimental material and isolated RhMYB23 (GenBank No. PQ441876) from its petals. The ORF of RhMYB23 was 771 bp and encoded 256 amino acids. Multiple sequence alignment and phylogenetic analysis revealed that RhMYB23 had a conserved R2R3 domain and was most closely related to Arabidopsis thaliana AtTT2 (transparent testa 2). Expression pattern analysis revealed that RhMYB23 was predominantly expressed in R. hybrida petals. RhMYB23 was localized in the nucleus and possessed transcriptional activation activity. Transient overexpression of RhMYB23 in R. hybrida petals significantly increased the accumulation of proanthocyanidins (P<0.05) and significantly upregulated the expression of key genes involved in proanthocyanidin biosynthesis, RhANR (anthocyanin reductase) and RhLAR (leucoanthocyanidin reductase) (P<0.001). These results suggested that RhMYB23 in R. hybrida might positively regulate proanthocyanidin synthesis. This study reveals the crucial regulatory role of RhMYB23 in proanthocyanidin biosynthesis in R. hybrida, offering theoretical support for R. hybrida flower color improvement.

Key words： Rosa hybrida RhMYB23 Flower color Proanthocyanidin

Received: 21 October 2024

ZTFLH:

S682

Corresponding Authors: *pingluo@zafu.edu.cn

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	FU Ting-Rui
	ZHANG Huan-Yu
	CUI Yong-Yi
	LUO Ping

Cite this article:

FU Ting-Rui,ZHANG Huan-Yu,CUI Yong-Yi, et al. Cloning and Functional Analysis of RhMYB23 in Rosa hybrida[J]. 农业生物技术学报, 2025, 33(8): 1674-1682.

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https://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2025.08.003 OR https://journal05.magtech.org.cn/Jwk_ny/EN/Y2025/V33/I8/1674

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