Analysis on the Basic Characteristics of the Effector Pt31812 of Puccinia triticina
WEN Xiao-Lei1,2, LI Jian-Yuan3, LI Ming-Yuan2, LI Hao1, ZHANG Na1, YANG Wen-Xiang1*
1 College of Plant Protection, Hebei Agricultural University/Technological Innovation Center for Biological Control of Plant Diseases and Insect Pests of Hebei Province/National Engineering Research Center for Agriculture in Northern Mountainous Areas, Baoding 071001, China; 2 Hebei Key Laboratory of Crop Stress Biology, Hebei Normal University of Science & Technology, Qinhuangdao 066600, China; 3 College of Bioscience and Bioengineering, Xingtai University, Xingtai 054000, China
Abstract Effectors play an important role in the process of pathogen invasion of plants. Understanding their functions is the key to analyzing the pathogenic mechanism of pathogenicity. In order to reveal the pathogenic mechanism of Puccinia triticina (Pt), based on the analysis of the biological information of effector Pt31812, the basic characteristics of the effector Pt31812 were analyzed using the secreting system of saccharase- deficient yeast, qRT-PCR and bacterial type Ⅲ secreting system. The results showed that Pt31812 CDS was 627 bp, encoding 208 amino acid, with 7 serine, 3 threonine and 2 tyrosine potential phosphorylation sites. The N-terminus has a hydrophobic structure, 1~20 amino acids were signal peptides, and had a secretory function, had no transmembrane domain, and did not act on mitochondria. It could inhibit the programmed cell death (PCD) induced by the Pseudomonas syringae pathovar. tomato strain DC3000 in wheat leaves. The gene began to express in the early stage of leaf rust infection, and the expression level was the highest at 96 and 36 h in the compatible and incompatible interactions, respectively. It could inhibit the deposition of callose and accumulation of reactive oxygen species in wheat both 'Thatcher' ('Tc') and 'TcLr1', as well as the expression of pathogenesis-related proteins gene 1 (TaPR1) and TaPR2, interfering with the host defense response. The results provide theoretical basis for further study of the pathogenic mechanism of wheat leaf rust and have guiding significance for durable resistant breeding.
WEN Xiao-Lei,LI Jian-Yuan,LI Ming-Yuan, et al. Analysis on the Basic Characteristics of the Effector Pt31812 of Puccinia triticina[J]. 农业生物技术学报, 2025, 33(6): 1181-1191.
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