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| Genome-wide Identification of PR-1 Gene Family in Capsicum annuum and Analysis of Its Expression Pattern in Response to Phytophthora capsici |
| HE Wei1, ZHANG Hui2*, WANG Jia-Lin1, HUANG Yue2, WANG Xing-Liang2, SHEN Xiao-Yu2 |
1 Department of Pharmacy, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, China;
2 Institute of Horticulture, Heilongjiang Academy of Agricultural Sciences, Harbin 150069, China |
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Abstract Pathogenesis-related protein 1 (PR-1), which are defense proteins in plant-pathogen interactions, play an important role in the resistance and defense of plants against diseases. The present study aimed at genome-wide identification and bioinformatics analyses of PR-1 genes in pepper (Capsicum annuum). The analyses resulted in the identification of 19 novel CaPR-1 genes, each of which produced a protein belonging to the CAP superfamily. Most of the CaPR-1 proteins contained an N-terminal signal peptide. Phylogenetic tree analysis of a total of 54 PR-1 protein sequences from pepper, tomato (Solanum lycopersicum) and Arabidopsis thaliana showed that the PR-1 family proteins were divided into 3 categories, and the pepper PR-1was closely related to tomato PR-1. The structural analysis revealed similar 3D structures of all CaPR-1 proteins except CaPR-1.1 and CaPR-1.12. In the promoter of CaPR-1 gene, hypothetical cis-elements related to defense stress response, plant hormones, light and transcription factor were detected. The GO and KEGG functional annotations predicted their function in the defense stress response, plant hormones and plant- pathogen interactions. Microarray data analysis showed that CaPR-1 not only responded to cold, salinity, drought and heat stresse, but also affected by exogenous hormones such as salicylic acid, methyl jasmonate, ethylene and abscisic acid. CaPR-1.4 and CaPR-1.15 genes were up-regulated under salinity or drought stress, and CaPR-1.17 gene was up-regulated inducted by 4 exogenous hormones. The expression pattern of CaPR-1 transcript was altered under infection with Phytophthora capsici, with up-regulation of alkaline CaPR-1 genes such as CaPR-1.4, CaPR-1.15 and CaPR-1.17. The drastic variation in the transcript levels of CaPR-1.17 gene was further validated through qRT-PCR and it showed a significant up-regulation in infected samples (P<0.05). The result showed that the CaPR-1 family might be important in plant stress resistance. This study provides a reference for in-depth exploration of the mechanism of pepper PR-1 family involved in P. capsic resistance.
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Received: 21 August 2024
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Corresponding Authors:
*la_jiao800@163.com
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