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| Isolation and Identification of Trichoderma spp. Antagonizing the Pathogen of Atractylodes macrocephala Blight Disease and Their Control Effect |
| WANG Jiao1, LIU Xiao-Ning1, JIAO Tian-Tian1, SHEN Feng-Ying2, LIU Da-Qun1, LI Ya-Ning1* |
1 College of Plant Protection/Innovation Center for Biological Control of Crop Diseases and Insect Pests of Hebei Province /National Engineering Research Center for Agriculture in Northern Mountainous Areas, Hebei Agricultural University, Baoding 071001, China;
2 Hebei North University, Zhangjiakou 075000, China |
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Abstract In recent years, due to continuous cropping, the problem of pests and diseases in Atractylodes macrocephala has been severe, resulting in a decrease in yield and a decline in quality. Among them, the A. macrocephala epidemic caused by mold has caused serious losses to A. macrocephala production. As one of the most important biocontrol fungi, Trichoderma spp. has good antagonistic effects on various plant pathogens and can produce various bioactive substances with antagonistic effects. In this study, healthy rhizosphere soil and underground rhizomes of A. macrocephala continuous cropping land were taken as materials, to screen antagonistic Trichoderma strains using the pathogen AMPH-1 of Phytophthora sansomeana as an indicator bacterium. Through the inhibitory effect and antagonistic level of antagonistic, 3 Trichoderma strains with better antibacterial effects, namely TPS-87, TPS-90, and TPS-99, were selected. The antibacterial rates against AMPH-1 were 77.19%, 80.04%, and 78.49%. With morphological and molecular identification, strain TPS-87 was determined as T. afroharzianum, while TPS-90 and TPS-99 were both T. atroviride. When applying the suspension of TPS-87, TPS-90, and TPS-99 with a concentration of 1×106 cfu/mL, and with the inoculation amount of 150 mL, all 3 strains of Trichoderma had a good promoting effect on A. macrocephala seedlings, and the greenhouse control effects on A. macrocephala blight disease were 61.54%, 53.85%, and 50%, respectively. This study provides biocontrol bacteria resources for the green prevention and control measures of A. macrocephala blight disease, and provides an important basis for the further development of biocontrol agents for the prevention and control of Chinese herbal medicine diseases.
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Received: 24 July 2024
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Corresponding Authors:
* yaning22@163.com
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