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| Cloning and Functional Analysis of NAC069 Gene in Canola (Brassica napus) |
| SHU Lin, LI Long-Hui, CAO Ya-Nan, WANG Ao-Min, YU Zhong-Chen, YAN Jing-Li* |
| College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, China |
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Abstract NAC (NAM, ATAF1/2, CUC2) transcription factors are a superfamily of transcription factors that are unique to plants and play an important role in plant development and stress response. In this study, BnaNAC069 was cloned from Brassica napus. Sequence analysis and phylogenetic analysis revealed that BnaNAC069 had a classical NAC domain in its N-terminal and a transmembrane domain in its C-terminal, which was a typical membrane-bound NAC transcription factor. BnaNAC069 had the closest genetic relationship with Arabidopsis thaliana AtNTL13/ANAC069. Subcellular localization showed that BnaNAC069 was located on the endoplasmic reticulum membrane. The expression pattern analysis showed that high salinity (NaCl), salicylic acid (SA) and hydrogen peroxide (H2O2) could significantly induce the expression of BnaNAC069. Phenotypic analysis showed that the A. thaliana anac069 mutants were tolerant to high salinity, while plants overexpressing BnaNAC069 were sensitive to drought and high salinity. The results of qRT-PCR showed that BnaNAC069 negatively regulated the expression of BnaRD29B (responsive to dehydration) and BnaAREB1 (ABA responsive element binding protein 1), suggesting that BnaNAC069 negatively modulates plant response to drought and high salinity in an ABA-dependent pathway. This study provides basic data for further investigation of the function of the BnaNAC069 gene in regulating plant response to drought and salt stress in B. napus.
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Received: 01 April 2024
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Corresponding Authors:
*yanjingli@henau.edu.cn
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