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| Comparison of the Size of Testes in Healthy Adult Male Duck (Anas platyrhynchos) and Analysis of the Expression of Related Regulatory Genes |
| ZHU Chun-Hong, TAO Zhi-Yun, WANG Zhi-Cheng, SONG Wei-Tao, LIU Hong-Xiang, ZHANG Shuang-Jie, XU Wen-Juan, GU Hao-Tian, WANG Yi-Fei, LI Hui-Fang* |
| Jiangsu Institute of Poultry Science, Yangzhou 225125, China |
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Abstract This study was aim to analyze the testis tissue from healthy adult male ducks (Anas platyrhynchos) with differences in testicular size and semen production by RNA-seq and bioinformatics. The differentially expressed genes (DEGs) and signaling pathways related to testicular size and spermatogenesis were screened to provide data for supporting male duck breeding. In this study, 40 male ducks (230-day-old) were used to collect testes tissues according to testis size (light weight of testes, GS; heavy weight of testes, GL) and semen production (less semen volume, GF; excessive semen volume, GM). Collect the testicular tissues and use the BGIseq platform for transcriptomic mRNA sequencing, GO and KEGG functional annotation and enrichment analysis were used to analyze the candidate DEGs. The expression of DEGs were verified by qRT-PCR. The results showed that there was a significant difference in testicular size among male ducks with no significant difference in body weight, there was also a significant difference in the production of semen after artificial training. A total of 383 DEGs were analyzed in different semen production and different testis size groups, and 36 DEGs were screened among different groups. The results of qRT-PCR showed that the result of transcriptome sequencing was accurate and reliable. GO and KEGG analysis showed that DEGs was significantly enriched in signal transduction, cell membrane, membrane composition, plasma membrane, G protein-coupled receptor activity, positive regulation of cell division and cAMP signal, pI3K-AKT signal, calcium signal pathway and other related pathways, genes GPR50, GPR132, GRER1, IGF1, TACR3, HTR1F, FGF19, FGF3, GABRG1, ADCY1 were enriched in these pathways, which might be closely related to testicular function. This study screened the genes related different testicular size and spermatogenic capacity in ducks, which provides a theoretical basis for the genetic breeding of testicular function.
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Received: 21 May 2024
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Corresponding Authors:
*lhfxf_002@aliyun.com
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[1] Birhanu Tesema.2017. 口服KISS1基因疫苗构建及其对绵羊生殖轴的影响[D]. 博士学位论文, 华中农业大学, 导师: 姜勋平, pp. 55-77.
(Birhanu Tesema.2017. Construction of an oral KISS1 gene vaccine and its effects on the reproductive axis of sheep[D]. Thesis for Ph.D., Huazhong Agricultural University, Supervisor: Jiang X P, pp. 55-77.)
[2] 陈艳茹, 李琦, 于娜, 等. 2023. 褪黑素通过PI3K/Akt信号通路调控支持细胞的增殖[J]. 中国兽医学报, 43(06):1327-1332.
(Chen Y R, Li Q, Yu N, et al.2023. Melatonin regulates the proliferation of supporting cells through the PI3K/Akt signaling pathway[J]. Chinese Journal of Veterinary Science, 43(06): 1327-1332.)
[3] 李海峰, 杜二球. 2022. 基于cAMP信号通路使用双酚A调节miRNA成熟对青春期鼠雄性生殖系统的影响[J]. 中国妇幼保健, 37(13): 2468-2471.
(Li H F, Du E Q.2022. Effects of bisphenol A on the maturation of miRNA mediated by the cAMP signaling pathway on the male reproductive system in adolescent mice[J]. Maternal and Child Health Care of China, 37(13): 2468-2471.)
[4] 李昆谕, 洪琼花, 陶林, 等. 2021. 山羊ADCY1和CYM基因多态性及其与产羔数关联分析[J]. 安徽农业科学, 49(24): 141-145+162.
(Li K S, Hong Q H, Tao L, et al.2021. Analysis of polymorphisms in the ADCY1 and CYM genes of goats and their association with litter size[J]. Journal of Anhui Agricultural Sciences, 49(24): 141-145+162.)
[5] 鲁生霞, 张德祥, 傅焕章, 等. 2008. 仿广西鸡公鸡睾丸生长发育的初步研究[J]. 养禽与禽病防治, (08): 12-16.
(Lu S X, Zhang D X, Fu H Z, et al. 2008. Preliminary study on the growth and development of the testes of Guangxi roosters[J]. Poultry Husbandry and Disease Control, (08): 12-16.)
[6] 罗静. 2020. 湖羊睾丸发育和大小相关基因的鉴定及SNPs的筛选[D]. 硕士学位论文, 兰州大学, 导师: 乐祥鹏, pp. 1-10.
(Luo J.2020. Identification of genes and SNPs related to testis development and size of Hu sheep[D]. Thesis for M.S., Lanzhou University, Supervisor: Le X P, pp. 1-10.)
[7] 马文萌. 2018. 黑线仓鼠大脑及哈氏腺中GPER1的繁殖调控效应[D]. 硕士学位论文, 曲阜师范大学, 导师: 徐金会, pp. 47-55.
(Ma W M.2018. Reproductive regulation effect of GPER1 in the brain and Harderian gland of Cricetulus barabensis[D]. Thesis for M.S., Qufu Normal University, Supervisor: Xu J H, pp. 47-55)
[8] 宋卫涛, 李慧芳, 徐文娟, 等. 2017. 苏邮1号蛋鸭精液品质评估[J]. 家畜生态学报, 38(05): 49-53.
(Song W T, Li H F, Xu W J, et al.2017. Assessment of semen quality in Suwei No.1 duck[J]. Journal of Domestic Animal Ecology, 38(05): 49-53.)
[9] 孙大业. 2001. 细胞信号转导[M]. 科学出版社, 北京.
(Sun D Y.2001. Cell Signal Transduction[M]. Science Press. Beijing.)
[10] 王华曦. 2014. bFGF与IGF1对大鼠睾丸间质细胞再生及睾酮分泌影响的初步研究[D]. 博士学位论文, 南方医科大学, 导师: 苏泽轩, pp. 109-122.
(Wang H X.2014. The preliminary study of bFGF and IGF1 on the effects of regeneration and testosterone secretio in leydig cells of rats[D]. Thesis for Ph.D., Southern Medical University, Supervisor: Su Z X, pp. 109-122.)
[11] 吴震, 朱文祥, 刘洪茂, 等. 2019. 热应激对睾丸钙/钙调蛋白依赖性蛋白激酶Ⅱ表达的影响[J]. 安徽医科大学学报, 54(12): 1938-1942.
(Wu Z, Zhu W X, Liu H M, et al.2019. Effects of heat stress on the expression of calcium/calmodulin-dependent protein kinase II in the testes[J]. Acta Universitatis Medicinalis Anhui, 54(12): 1938-1942.)
[12] 薛敏开, 宋卫涛, 朱春红, 等. 2017. “苏邮1号”成年公鸭睾丸发育与精液品质的相关性分析[J]. 中国家禽, 39(14): 68-70.
(Xue M K, Song W Z, Zhu C H, et al.2017. Analysis of the correlation between testis development and semen quality in adult male "Suyou 1" ducks[J]. China Poultry, 39(14): 68-70.)
[13] 闫秀文. 2015. bFGF对大鼠骨髓间充质干细胞向睾丸间质细胞分化的作用研究[D]. 硕士学位论文, 山西医科大学, 导师: 刘春, pp. 17-20.
(Yan X W.2015. The role of bFGF on the differentiation from rat bone marrowmesenchymal stem cells to the leydig cells[D]. Thesis for M.S., Shanxi Medical University, Supervisor: Liu C, pp. 17-20.)
[14] 苑志宇, 董杨云逸, 李锰, 等. 2019. 不同光照周期对鸡睾酮合成的影响[J]. 中国畜牧杂志, 55(03): 99-102.
(Yu Z Y, DongYang Y Y, Li M, et al.2019. The effects of different lighting cycles on testosterone synthesis in chickens[J]. Chinese Journal of Animal Science, 55(03): 99-102.)
[15] 曾圣. 2012. FGF家族生物信息学分析及FGF9/16/20亚家族在罗非鱼性腺发育中的可能作用[D]. 硕士学位论文, 西南大学, 导师: 王德寿, pp. 1-7.
(Zeng S.2012. The bioinformatical analysis on FGF family and possible role of FGF9/16/20 subfamily in the development of tilapia gonad[D]. Thesis for M.S., Southwest University, Supervisor: Wang D S, pp. 1-7.)
[16] 张意茗. 2015. 特发性低促性腺激素性性腺功能减退症致病基因筛选及IVF妊娠结局分析[D]. 博士学位论文, 山东大学, 导师: 陈子江, pp. 20-33.
(Zhang Y M.2015. Screening of causative genes in Chinese patients with idiopathic hypogonadotropic hypogonadism (IHH) and the reproductive performance of women with IHH during in vitro fertilization and embryo transfer treatment[D]. Thesis for Ph.D., Shandong University, Supervisor: Chen Z J, pp. 20-33.)
[17] 周虚. 1990. 成年雄性大鼠睾丸大小及其与体重的关系[J]. 兽医大学学报, (01): 60.
(Zhou X. 1990. Size of the testes in adult male rats and its relationship with body weight[J]. Chinese Journal of Veterinary Science, (01): 60.)
[18] Azizi H, Asgari B, Skutella T.2019. Pluripotency potential of embryonic stem cell-like cells derived from mouse testis[J]. Cell Journal, 21(3): 281-289.
[19] Biggs K, Seidel J S, Wilson A, et al.2013. gamma-Amino-butyric acid (GABA) receptor subunit and transporter expression in the gonad and liver of the fathead minnow (Pimephales promelas)[J]. Comparative Biochemistry and Physiology. Part A, Molecular and Integrative Physiology, 166(1): 119-127.
[20] Foster R A, Ladds P W, Hoffmann D, et al.1989. The relationship of scrotal circumference to testicular weight in rams[J]. Australian Veterinary Journal, 66(1): 20-22.
[21] Liu X, Herbison A E.2013. Dopamine regulation of gonadotropin-releasing hormone neuron excitability in male and female mice[J]. Endocrinology, 154(1): 340-350.
[22] Ouyang Q, Bao D, Lu Y, et al.2021. A comparative study of libido in drakes: From phenotypes to molecules[J]. Poultry Science, 100(12): 101503.
[23] Ran M L, Chen B, Wu M S.2015. Integrated analysis of miRNA and mRNA expressionprofiles in development of porcine testes[J]. RSC Advances, 5(78): 63439-63449.
[24] Rochester J R, Chung W C, Hayes T B, et al.2012. Opposite-sex housing reactivates the declining GnRH system in aged transgenic male mice with FGF signaling deficiency[J]. American Journal of Physiology. Endocrinology and Metabolism, 303(12): E1428-1439.
[25] Song H, Zhu L, Li Y, et al.2015. Exploiting RNA-sequencing data from the porcine testes to identify the key genes involved in spermatogenesis in large white pigs[J]. Gene, 573(2): 303-309.
[26] Sozubir S, Barber T, Wang Y, et al.2010. Loss of Insl3: A potential predisposing factor for testicular torsion[J]. Journal of Urology, 183(6): 2373-2379.
[27] Tillakaratne N J, Medina-Kauwe L, Gibson K M.1995. gamma-Aminobutyric acid (GABA) metabolism in mammalian neural and nonneural tissues[J]. Comparative Biochemistry and Physiology A: Physiology, 112(2): 247-263.
[28] Ullah A, Jahan S, Razak S, et al.2017. Protective effects of GABA against metabolic and reproductive disturbances in letrozole induced polycystic ovarian syndrome in rats[J]. Journal of Ovarian Research, 10(1): 62.
[29] Yarney T A, Sanford L M.1993. Pubertal development of ram lambs: Physical and endocrinological traits in combination as indices of postpubertal reproductive function[J]. Theriogenology, 40(4): 735-744.
[30] Zhang L, Wang H, Yang Y, et al.2013. NGF induces adult stem Leydig cells to proliferate and differentiate during Leydig cell regeneration[J]. Biochemical and Biophysical Research Communications, 436(2): 300-305.
[31] Zhang M, Zou XT, Li H, et al.2012. Effect of dietary gamma-aminobutyric acid on laying performance, egg quality, immune activity and endocrine hormone in heat-stressed Roman hens[J]. Animal Science Journal, 83(2): 141-147. |
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