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| Effects of Waste Residue of Cinnamomum longepaniculatum on the Cultivation of Pinellia ternata and Microorganisms of Rhizospheric Soil |
| WANG Yan-Zhi, XIONG Lin, ZHANG Jian*, WANG Li, LI Ya, WU Yan-Fang |
| Faculty of Agriculture, Forestry and Food Engineering, Yibin University, Yibin 644000, China |
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Abstract The soil physicochemical properties, microbial community structure, and functional groups of artificially cultivated Pinellia ternata have undergone changes, resulting in low yield and unstable quality. However, the processing residue of Cinnamomum longepaniculatum is rich in organic matter and antibacterial components. Therefore, this study applied the waste residue of C. longepaniculatum to the cultivation of P. ternata in order to improve the yield and quality of P. ternata and realize the sustainable utilization of C. longepaniculatum. In this study, 3 treatment groups were set: The whole soil (CK), waste residue∶soil=3∶1 (V/V)(T6), the whole waste residue (T8), which began on March 15, 2022 and ended on November 15, 2022. The growth indicators of P. ternata were measured on April 15 and May 1, and the physiological indicators of P. ternata were measured on May 15. The disease situation of P. ternata was statistically analyzed throughout the process. After P. ternata fell, metagenome platform was used to conduct sequencing studies on the differences of rhizospheric microbial community and function, and determined the yield of P. ternata. The results showed that T6 and T8 both increased the number of seedlings, seedling height, leaf length and width of P. ternata, increased its protein content, malondialdehyde content, peroxidase activity, and proline content, but decreased its SPAD (soil and plant analyzer development) value and superoxide dismutase activity; T6 treatment could alleviate the disease of P. ternata, but T8 treatment was more serious; The yield of T6 treatment increased, but the yield of T8 treatment decreased; The proportion of bacteria in P. ternata rhizosphere soil (CK) was very high (96.32%~99.57%), while the proportion of fungi was very low (0.27%~0.82%). The number of bacteria in the T6 and T8 groups increased by 3.16% and 3.25% compared to CK, respectively, while the number of fungi decreased by 0.41% and 0.55%, respectively; After adding waste residue of C. longepaniculatum, the relative abundance of beneficial bacteria Proteobacteria, Steroidobacter, Povalibacter, Steroidobacter, Agromyces and Conexibacter increased. The relative abundance of harmful bacteria Acidobacteria was decreased; The abundance of COG (clusters of homologous groups of proteins) functional genes in the T6 and T8 groups increased, which was closely related to the genera Agromyces, Conexibacter, and Streptomyces. In summary, waste residue of C. longepaniculatum could improve the soil condition of P. ternata, and the T6 treatment group had a better effect. This study provides theoretical basis for the utilization of waste residue of C. longepaniculatum in P. ternata cultivation.
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Received: 03 May 2023
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Corresponding Authors:
* zhangjian840000@163.com
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