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| Effects of Engineered Soil Improvement Combined with Biofertilizer on Rhizosphere Soil Microbial Community in Replanted Apple (Malus domestica) |
| JIANG Ting-Ting1, REN Zhong-Xiu2, LIU Liang1, YU Jia-Yi2*, YUAN Hong-Li1* |
1 College of Biological/State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing 100193, China;
2 Beijing Siliang Technology Limited Company, Beijing 100101, China |
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Abstract Apple (Malus domestica) continuous cropping obstacle hinders the development of apple industry significantly, which inhibits the survival rate of apple seedlings, and lowers the yield and quality of apple seriously. In order to explore the effect of engineering soil improvement combined with biological fertilizer technology improvement on rhizosphere microbial community of replanted apple and the controlling and the apple rhizosphere soil samples after 1 and 2 years of engineered soil improvement from loam orchards were collected in Guandao Town, Qixia, Shandong Province. The effects of engineering soil modification on apple continuous cropping obstacle soil were investigated from the perspectives of microbial community composition and network stability by high-throughput sequencing of 16S rRNA and intergenic transcribed spacer (ITS) genes. The results showed that the α diversity of bacteria community was increased and community structure was changed significantly after soil improvement. At phylum level, the relative abundance of Proteobacteria and Actinobacteria were increased, while the abundance of Acidobacteria was decreased. At genus level, the abundance of Pseudomonas and Massilia were increased continuously. Arthrobacter, Streptomyces, Acinetobacter, Sphingobium and Williamsia were enriched significantly during the process of soil improvement. The α diversity of fungi was not affected obviously, however, the community structure of fungi was changed. At phylum level, the relative abundance of Ascomycota, Basidiomycota and Mortierellomycota were increased. At genus level, the abundance of Fusarium, Penicillium and Cladosporium were decreased, while Mortierella and Paraphaeosphaeria were enriched significantly. Meanwhile, the co-
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Received: 22 August 2022
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Corresponding Authors:
*leonayu2000@126.com; hlyuan@cau.edu.cn
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