Medium Selection in Tissue Culture of Nipponbare Scutellum and Obtainment Regeneration of Transgenic Plants with Elicitor-coding Gene from Magnaporthe grisea
Abstract：N6 medium, MS medium and BN medium were adopted in tissue culture of Nipponbare (Japanica) Scutellum for performance comparison. Results indicated that, among the three kinds of culture mediums, NB medium worked best in the calli induction and gene transformation and was most suitable for tissue culture of Nipponbare scutellum. Based on this, elicitor-encoding gene pemG1 from Magnaporthe grisea was introduced into the genome of Nipponbare via Agrobacterium-mediated method. Transgenic rice plants were obtained. The integration, transcription and expression of pemG1 in rice were respectively confirmed by PCR, Northern blot and Western blot. Genetic analysis showed that the transgenes were segregated normally in the progenies.
毛建军 曾洪梅 杨秀芬 袁京京 邱德文. 日本晴组培培养基的筛选及转稻瘟菌蛋白激发子基因植株的获得[J]. , 2008, 16(5): 0-.
. Medium Selection in Tissue Culture of Nipponbare Scutellum and Obtainment Regeneration of Transgenic Plants with Elicitor-coding Gene from Magnaporthe grisea. , 2008, 16(5): 0-.
