Cloning and Functional Analysis of GmSDIR1 Gene in Soybean (Glycine max)
ZHU Gui-Shuang1,3, ZHAO Ying2, WANG Xiao-Dong1, ZHANG Wei-Wei1, XIANG Dian-Jun1,*, LI Zhi-Gang1,*
1 Agricultural College, Inner Mongolia University for Nationalities, Tongliao 028000, China; 2 Tongliao Agriculture and Animal Husbandry Development Center, Tongliao 028000, China; 3 Tongliao Academy of Agricultural and Animal Husbandry Sciences, Tongliao 028015, China
Abstract：Salt- and drought-induced ring finger protein (SDIR) plays an important role in abscisic acid (ABA)-mediated plant stress response, and SDIR is rarely reported in soybean (Glycine max). In this study, the candidate gene GmSDIR1 was screened and cloned from soybean by co-expression analysis of transcriptome sequencing data and weighted gene co-expression network analysis (WGCNA). It was located on chromosome 11, with a full-length coding region of 825 bp, belonging to C3H2C3 type RING finger protein. Subcellular localization showed that GmSDIR1 protein was localized in the nucleus. The expression pattern analysis showed that GmSDIR1 gene was expressed in true leaf, cotyledon and stem tissues of soybean and had spatial and temporal expression specificity. High salt (NaCl), drought (PEG6000), abscisic acid (ABA) and low temperature (4 ℃) stress treatments could significantly induce the expression of GmSDIR1 gene.Phenotypic analysis showed that tobacco (Nicotiana tobacum) overexpressing GmSDIR1 gene was tolerant to high salt stress, and the heterologous expression of GmSDIR1 promoted seed germination, root length elongation and fresh weight increase of seedlings, and improved the antioxidant capacity of transgenic tobacco. The results of qRT-PCR showed that the expression of GmSDIR1 in transgenic tobacco was significantly activated under high salt stress, which indicated that GmSDIR1 might be involved in the regulation of plant response to salt stress. This study provides an important experimental basis for further analysis of the molecular mechanism of soybean GmSDIR1 gene regulating plant high salt stress response.
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