Application of Serum Starvation Strategy in the Treatment of Canine (Canis lupus familiaris) Diabetes with Mesenchymal Stem Cells
DU Jia-Xin1*, LI Ba-Lun2*, KOU Zheng2, WU Li-Ping1, KONG Xue-Li1, ZHAO Xian-Jun2, HUA Jin-Lian**
1 Department of Animal Engineering, Yangling Vocational & Technical College, Yangling 712100, China;
2 College of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering & Technology, Northwest A&F University, Yangling 712100, China
Abstract：Mesenchymal stem cells (MSCs) have the characteristics of promoting tissue repair, activating cytokine secretion, regulating organism immunity, and low moral and ethical constraints, which can provide effective treatment for various complex diseases in veterinary clinic. At present, MSCs are often cultured in a medium containing 10% fetal bovine serum (FBS), and the high cost of serum limits the mass production and application of MSCs. In this study, MSCs with a density of 70% were treated with α-MEM medium with gradient FBS of 0.5%~10% respectively. Cells were collected at 6, 12, 18, 24 and 48 h for Giemsa staining, EDU (5 ethynyl-2' deoxyuridine) staining and cell migration experiments, respectively. The results showed that Serum Starvation (SS) of 2% FBS for 6 h could promote the migration ability of MSCs in vitro. Twelve diabetic dogs (Canine lupus familiaris) were given 1×107 MSCs and SS-MSCs by intravenous injection, and the changes of blood sugar and weight of dogs were monitored within 2 weeks. Serological examination and histological examination were carried out 2 weeks after stopping treatment. The results showed that SS-MSCs group achieved similar therapeutic effects to normal culture environment, including restoring blood sugar and weight of diabetic dogs, remodeling islets, and improving liver glucose metabolism. This study provides a feasible strategy for clinical large-scale preparation of medical-grade MSCs, and improves the application value of MSCs in clinical disease treatment.
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