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2025年4月30日 星期三
农业生物技术学报  2024, Vol. 32 Issue (4): 751-761    DOI: 10.3969/j.issn.1674-7968.2024.04.002
  研究论文与报告 本期目录 | 过刊浏览 | 高级检索 |
转录组分析调控桃采后冷害和硬度的候选主效基因
张志刚1,2, 陈玉峰2, 王长江2, 徐晋2, 姜延伟2, 郑亚琴1, 刘振宁1, 陈守兵1, 刘一华1,*, 徐蒙1,*
1 临沂大学 农林科学学院,临沂 276000;
2 邹城市自然资源和规划局,济宁 273500
Transcriptome Analysis of the Candidate Key Genes Regulating Post-harvest Chilling Injury and Firmness in Peach (Prunus persica)
ZHANG Zhi-Gang1,2, CHEN Yu-Feng2, WANG Chang-Jiang2, XU Jin2, JIANG Yan-Wei2, ZHENG Ya-Qin1, LIU Zhen-Ning1, CHEN Shou-Bing1, LIU Yi-Hua1,*, XU Meng1,*
1 College of Agriculture and Forestry Sciences, Linyi University, Linyi 276000, China;
2 Zoucheng Natural Resources and Planning Bureau, Jining 273500, China
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摘要 冷敏性果实采后低温贮藏容易造成果实冷害,程序性降温(low-temperature conditioning, LTC)处理在减缓桃(Prunus persica)等冷敏性果实冷害中具有显著效果,但是贮藏前期果实会发生轻微软化。本研究通过加权基因共表达网络分析(weighted gene co-expression network analysis, WGCNA)发现了与冷害和硬度表型高度相关的基因模块。分别对模块基因进行主效转录因子鉴定和调控网络构建,发现桃WRKY转录因子PpWRKY43和生长素响应因子(auxin response factor, ARF) PpARF1可能通过调控冷调节蛋白(cold-regulated protein, COR) PpCOR413-2和脂氧合酶 (lipoxygenase 5, LOX5) PpLOX5参与果实采后冷害调控,NAC转录因子PpNAC5可能通过调控桃果胶裂解酶2 (P. persica pectate lyase 2, PpePL2)参与桃果实采后软化。本研究结果为桃果实采后保鲜减损研究提供基础资料。
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张志刚
陈玉峰
王长江
徐晋
姜延伟
郑亚琴
刘振宁
陈守兵
刘一华
徐蒙
关键词 采后冷害加权基因共表达网络分析(WGCNA)硬度    
Abstract:Cold-sensitive fruit stored at low temperatures after harvest is vulnerable to fruit chilling injury, and low-temperature conditioning (LTC) treatment has notable effect in alleviating chilling injury of cold-sensitive fruit such as peach (Prunus persica), but slight fruit softening occurs in the initial stage of storage. In this study, weighted gene co-expression network analysis (WGCNA) was used to identify gene modules that were highly correlated with chilling injury and firmness phenotypes. The analysis revealed that WRKY transcription factor PpWRKY43 and P. persica auxin response factor 1 (PpARF1) might be involved in postharvest chilling injury regulation by regulating cold-regulated 413 inner membrane protein 2 (PpCOR413-2) and the lipoxygenase 5 (PpLOX5), and NAC transcription factor PpNAC5 might be involved in postharvest softness of peach fruit by regulating P. persica pectate lyase 2 (PpePL2). The results of this study provides basic data for postharvest preservation and reduction of peach fruit injury.
Key wordsPeach    Postharvest    Chilling injury    Weighted gene co-expression network analysis (WGCNA)    Firmness
收稿日期: 2023-05-20     
ZTFLH:  S662.1  
基金资助:山东省自然科学基金(ZR2021QC100); 山东省现代农业产业技术体系(SDAIT-06-08); 山东省高等学校青创科技计划创新团队(2021KJ055)
通讯作者: *liuyihua@lyu.edu.cn;xumeng@lyu.edu.cn   
引用本文:   
张志刚, 陈玉峰, 王长江, 徐晋, 姜延伟, 郑亚琴, 刘振宁, 陈守兵, 刘一华, 徐蒙. 转录组分析调控桃采后冷害和硬度的候选主效基因[J]. 农业生物技术学报, 2024, 32(4): 751-761.
ZHANG Zhi-Gang, CHEN Yu-Feng, WANG Chang-Jiang, XU Jin, JIANG Yan-Wei, ZHENG Ya-Qin, LIU Zhen-Ning, CHEN Shou-Bing, LIU Yi-Hua, XU Meng. Transcriptome Analysis of the Candidate Key Genes Regulating Post-harvest Chilling Injury and Firmness in Peach (Prunus persica). 农业生物技术学报, 2024, 32(4): 751-761.
链接本文:  
https://journal05.magtech.org.cn/Jwk_ny/CN/10.3969/j.issn.1674-7968.2024.04.002     或     https://journal05.magtech.org.cn/Jwk_ny/CN/Y2024/V32/I4/751
 
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