Abstract：Nanobodies are small molecules composed of only heavy chains with specific recognition of antigens, which have broad application prospects. In this paper, three different antibody subtypes in camels (Camelus) were introduced, and the similarities and differences between traditional antibodies and nanobodies in the types and positions of disulfide bonds, the composition of skeleton regions, and complementary determining regions were revealed. The characteristics of nanobodies, such as simple production, strong specificity, high stability, and high solubility, were discussed. The screening and expression methods of nanobodies were expounded, that is, phage display, yeast two-hybrid and other technologies can be used to screen and obtain specific nanobodies. Because of its simple structure, it can be expressed in Escherichia coli and yeast (Saccharomyces) and other organisms, to better study its function. This review provides a theoretical basis for the development and application of nanobodies in the future.
孔静, 郑楠, 王加启, 赵圣国. 纳米抗体结构特征与制备研究进展[J]. 农业生物技术学报, 2024, 32(1): 209-219.
KONG Jing, ZHENG Nan, WANG Jia-Qi, ZHAO Sheng-Guo. Research Progress on Structural Characteristics and Preparation of Nanobodies. 农业生物技术学报, 2024, 32(1): 209-219.
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