Effects of Sex and Age on Inflammation-related Genes Expression in Omental Adipose Tissue of Holstein Cattle (Bos taurus) Based on Transcriptomics
CHAI Jin-Bao1,2, LIU Li1,*, WANG Fang1, YANG Shuo1, CAO Pei-Li1, ZHAO Xiao-Chuan1, XU Shan-Shan1, MENG Xiang-Ren1, BU Ye1, YUE Meng-Meng2, WU Rui2*, SUN Fang1
1 Key Laboratory of Combining Farming and Animal Husbandry of Ministry of Agriculture, Institute of Animal Husbandry, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China; 2 College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, China
Abstract：Castration and long term fattening of bulls (Bos taurus) could promote visceral fat deposition, excessive fat deposition was easy to cause systemic chronic inflammation. However, there was very little known molecular mechanism of omental adipose tissue inflammation in cattle. The present study selected omental adipose tissue of cattle as research object, the aim of the present study was to evaluate the effects of sex and age on inflammation-related genes expression in omental adipose tissue of Holstein fattening cattle. Holstein bulls at 16 months of age (BF16 group, n=3) and Holstein steers at 16 and 26 months of age (SF16 and SF26 group, n=3) were selected. The growth performance and blood biochemical indexes were measured. After slaughtering, omental adipose tissue samples were collected for hematoxylin-eosin staining (HE) staining, transcriptome analysis was carried out for omental adipose tissue from three groups cattle by RNA- seq. Differentially expressed genes (DEGs) between groups was screened, GO function annotation, KEGG enrichment analysis, qPCR validation, candidate gene screening and protein-protein interaction network of differentially expressed genes were conducted. The results showed that a total 1 173 differentially expressed genes were identified, and enriched in 8 gene expression trend profiles. Functional analysis showed that the differentially genes were involved in multiple biological processes and signal pathways related to inflammation. Six differentially expressed genes were randomly selected for qPCR validation, and the results were consistent with transcriptome sequencing, which indicated that the sequencing results were reliable. Fifty non-redundant genes in 5 biological process items and 5 signaling pathways related to inflammation were screened. Cluster analysis showed that castration could upregulate the expression of anti-inflammatory and adipocyte differentiation genes (such as TNF alpha induced protein 3 (TNFAIP3), bone morphogenetic protein 2 (BMP2), etc) and downregulate the expression of proinflammatory genes (such as interleukin-1beta (IL1B)) in omental adipose tissue of SF16 group in comparison with BF16 group, long term fattening could upregulate the expression of genes related to aging and chronic inflammation (such as galectin 3 (LGALS3), C-C motif chemokine receptor 7 (CCR7), etc)) in omental adipose tissue of SF26 group in comparison with BF16 and SF16 groups. Protein-protein interaction network analysis showed that IL1B was the key central node. This research can serve as a theoretical basis for optimizing marbled beef production technology for Holstein steer.
柴金宝, 刘利, 王芳, 杨硕, 曹培丽, 赵晓川, 许珊珊, 孟详人, 卜也, 岳萌萌, 武瑞, 孙芳. 基于转录组学分析性别、月龄对荷斯坦牛网膜脂肪组织炎症相关基因表达的影响[J]. 农业生物技术学报, 2023, 31(5): 901-913.
CHAI Jin-Bao, LIU Li, WANG Fang, YANG Shuo, CAO Pei-Li, ZHAO Xiao-Chuan, XU Shan-Shan, MENG Xiang-Ren, BU Ye, YUE Meng-Meng, WU Rui, SUN Fang. Effects of Sex and Age on Inflammation-related Genes Expression in Omental Adipose Tissue of Holstein Cattle (Bos taurus) Based on Transcriptomics. 农业生物技术学报, 2023, 31(5): 901-913.
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