Strain Screening, Combinations Preparation and Biocontrol Efficacy of Antagonistic Streptomyces spp. Targeting Potato Common Scab
CHEN Chen1, ZHANG Cai-Hua1, WANG Shi-Yi3, ZHANG Hao-Yu3, DING Yu2, LEI Bo-Chao2, SUN Man-Li2, ZHAO Wei-Quan2,*, YU Xiu-Mei1,*
1 College of Life Sciences, Hebei Agricultural University/Key Laboratory of Hebei Province for Plant Physiology and Molecular Pathology, Baoding 071001, China; 2 College of Plant Protection, Hebei Agricultural University/Innovation Center of Biological Control Technology for Crop Diseases and Pests, Baoding 071001, China; 3 Hebei Jiuen Agricultural Development Co., Ltd., Zhangjiakou 076550, China
Abstract：Potato (Solanum tuberosum) common scab, caused by pathogenic Streptomyces spp., is an important soil-borne disease that occurs worldwide. Non-pathogenic Streptomyces can produce a large number of metabolites with antimicrobial and growth-promoting effects, making them one of the microorganisms with the greatest biocontrol potential. To obtain non-pathogenic Streptomyces strains with biocontrol potential against Streptomyces scabies which causes potato common scab, this study conducted series of assays, including screening for antimicrobial activity of single strain in vitro, preparation for antagonistic Streptomyces combinations, testing for antimicrobial activity of the combinations, molecular identification of strains included in the different combinations, analysis for their antimicrobial and growth-promoting characteristics, and evaluation for biocontrol efficacy in greenhouse. The results showed that 15 strains with significant antagonistic effects against the S. scabies strain LBX-7 were obtained from 77 non-pathogenic Streptomyces strains. Among them, strain No. ZL21 exhibited the widest inhibition band (8.17 mm). After strain compatibility testing and combinations preparation, 3 combinations with antagonistic effects against LBX-7 were obtained. Among these, combination No. C (ZL21\ZL109\ZL144) showed the best antimicrobial effect, with an inhibition band of 7.67 mm in width. Multi-gene sequencing were used to identify the strains in the 3 combinations, strains No. ZL21 and ZL144 belonged to S. olivaceus, while strains ZL54, ZL68, ZL79, ZL90, ZL109 and 05B3 were identified as S. parvus. All strains in the combinations had the abilities to produce protease and chitinase, decompose potassium and fix nitrogen, but none of them could dissolve inorganic phosphorus. Several strains were capable of producing indole-3-acetic acid, cellulase, laccase and amylase. Greenhouse pot control experiment indicated that ZL21 exhibited the highest biocontrol efficacy (75.2%) in all the tested single strains, and combination No. C showed the best efficacy (54.4%) among the 3 combinations in greenhouse. This study obtained candidate Streptomyces strains ZL21 with good biocontrol efficacy against potato common scab. This strain exhibits promising potential as resources for controlling potato common scab.
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