Abstract：Highly intensive breeding of mutton sheep (Ovis aries) can easily lead to oxidative stress, reduce body resistance, disturb gastrointestinal flora, and affect growth performance. Dietary bacterial enzyme compound preparation can improve intestinal flora structure, nutrient digestibility and animal growth performance. This study aimed to investigate the effects of bacterial enzyme compound preparation on growth performance, serum indexes and fecal microorganisms of Hu sheep. A total of 48 male Hu lambs ((18.00±2.00) kg) aged 2 months were randomly assigned into 4 groups. Lambs in Group C (control) were fed a basal diet, and the other 3 groups were fed a basal diet with 0.1% (Group 1), 0.2% (Group 2) and 0.3% (Group 3) bacterial enzyme preparation, respectively. The feeding included a pre-experiment for 7 d and a formal experiment for 60 d. Results showed that, compared with Group C, average daily gain (ADG) in Group 1 and Group 2 increased significantly (P<0.05), and the feed to gain ratio (F/G) significantly decreased in all groups (P<0.05) during 31~60 d and 1~60 d; serum albumin ( ALB) and glucose (GLU) content in all groups and high density lipoprotein in Group 1 increased significantly (P<0.05); serum activity of superoxidized dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) increased significantly in all groups (P<0.05); serum immunoglobulin (Ig) IgA and IgG increased significantly in all groups (P<0.05), and serum IgM increased significantly in Group 3 (P<0.05); the apparent digestibility of neutral detergent fibre (NDF) and acid detergent fibre (ADF) in Group 2 significantly increased (P<0.05). At phylum level, the abundance of Verrucomicrobiota increased significantly (P<0.05), while Cyanobacteria and Campylobacterota decreased significantly (P<0.05). At genus level, the abundances of Alistipes and Prevotellaceae_UCG-001 significantly increased (P<0.05), while Candidatus_Saccharimonas significantly decreased (P<0.05). In conclusion, the bacterial enzyme compound preparation could improve the growth performance, antioxidant and immune capacity, improve nutrient apparent digestibility, promote the colonization of beneficial bacteria, inhibit the colonization of harmful bacteria. The addition effect of 0.2% bacterial enzyme compound preparation was the best. This study provides a scientific basis for the application of bacterial enzyme compound preparation in the production of Hu sheep.
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