Screening, Identification and Biological Characteristics Research of Digestive Enzyme Secreting Probiotics from Gibel Carp (Carassius auratus gibelio) CASⅤ
YU Peng1, MA Jing-Jing1,*, CHENG Yu1, ZHANG Cheng-Shuo1, WANG Kai1, ZHOU Zheng-Hai2
1 Jiangsu Coastal Area Institute of Agricultural Sciences, Yancheng 224001, China; 2 Jiangsu Zheng Yuan Chuang Hui Agricultural Science and Technology Development Co., Ltd., Yancheng 224700, China
Abstract：The low feed digestibility and absorption rate is an important reason for high feed cost and low profit of fish farming. Gibel carp (Carassius auratus gibelio) is one of the main current crucian carp breeding in China, the aim of this study was to screen safe and efficient probiotics which can secret digestive enzymes from healthy culture system of gibel carp CASⅤ, so as to improve feed digestion and absorption capacity of CASⅤ and reduce feed cost. Ten strains with protease, lipase and amylase activities were isolated from CASⅤ and pond water by spot inoculation method. Two highly efficient, non-hemolytic, sensitive to most antibiotics and non-pathogenic strains, ZKW7 and ZKF17, were obtained by further analysis of enzyme secretion ability and safety test (hemolysis, antibiotics susceptibility and intraperitoneal infection). Based on 16S rDNA sequence analysis, both strains had the highest similarity to Bacillus subtilis and B. halotolerans. The results of biological characteristics analysis showed that both strains had strong degrading activities for NH4+, NO2- and NO3-. The optimum growth conditions of ZKW7 were pH 8, salinity 15 and Fe2+ in culture medium, respectively. By contrast, the optimum growth conditions of ZKF17 were pH 7, salinity 10~30 and the presence of Mg2+. In addition, these 2 strains were resistant to acid, bile salt, intestinal fluid and succus gastricus to some extent. In the present study, 2 strains of safe and efficient enzyme-producing Bacillus sp. were obtained, which could be used as potential probiotics and would be conducive to the healthy farming of C. auratus gibelio var. CASⅤ.
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