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2025年4月5日 星期六
农业生物技术学报  2024, Vol. 32 Issue (10): 2243-2254    DOI: 10.3969/j.issn.1674-7968.2024.10.004
  研究论文与报告 本期目录 | 过刊浏览 | 高级检索 |
红麻基因组WRKY家族成员的鉴定及其在镉胁迫下的表达分析
李辉1,2, 陈安国2, 唐慧娟2, 栾明宝2,3,*
1 湖南文理学院 生命与环境科学学院,常德 415000;
2 中国农业科学院 麻类研究所,长沙 410205;
3 三亚中国农业科学院国家南繁研究院,三亚 572000
Identification of Kenaf (Hibiscus cannabinus) WRKY Family Member and Analysis of Their Expression Under Cadmium Stress
LI Hui1,2, CHEN An-Guo2, TANG Hui-Juan2, LUAN Ming-Bao2,3,*
1 College of Life and Environmental Sciences, Hunan University of Arts and Science, Changde 415000, China;
2 Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, China;
3 National Nanfan Research Institute (Sanya), Chinese Academy of Agricultural Sciences, Sanya 572000, China
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摘要 重金属镉污染农田的修复是世界性的难题。红麻(Hibiscus cannabinus)耐逆性强,可用于镉污染农田的修复。为了探索红麻WRKY家族成员对镉胁迫的响应模式,本研究利用HMMER和BLAST软件在红麻基因组中鉴定获得HcWRKY家族成员,并通过ExPasy在线网站获得了HcWRKY家族成员的分子信息,同时利用TBtools v1.6软件将家族成员全部定位到相应染色体上。结果表明,33个HcWRKY家族成员不均匀地分布在12条染色体上,其理化性质,如氨基酸数量、蛋白质分子量和理论等电点各不相同。除HcWRKY4-2位于细胞质外,其他HcWRKY成员均定位于细胞核内。系统发育分析表明,33个HcWRKY蛋白分为4组:其中第Ⅱ组和第Ⅳ组仅含HcWRKY家族成员;第Ⅰ组和Ⅲ组同时含有HcWRKY和AtWRKY家族成员。实时荧光定量PCR分析表明,受镉胁迫诱导表达的HcWRKY家族成员有24个。在红麻响应镉胁迫反应过程中22个HcWRKY家族成员基因表达量上调,2个HcWRKY家族成员基因表达量下调。这可能是红麻耐镉能力强的一个重要因素。本研究结果为进一步探索HcWRKY基因家族在红麻响应镉胁迫过程中的生物学功能提供了基础资料。
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李辉
陈安国
唐慧娟
栾明宝
关键词 红麻镉胁迫WRKY基因家族    
Abstract:It is a global challenge that the remediation of cadmium contaminated farmland. Kenaf (Hibiscus cannabinus) can be used to remediate Cd-contaminated farmland, as it has strong stress resistance. In order to explore the response patterns to Cd stress of HcWRKY gene family in kenaf, in this study, HMMER and BLAST software were used to identify HcWRKY family genes in genome-wide. The ExPasy website was used to obtain the molecular information of the HcWRKY members. The HcWRKY members were mapped to kenaf chromosomes using TBTOOLS v1.6 software. The results suggested that 33 HcWRKY family members were unequally scattered on 12 chromosomes. It was difference that the physical and chemical properties, such as the number of amino acids, molecular weight, and theoretical isoelectric point in each HcWRKY family member. Excepting the HcWRKY4-2 located in cytoplasm, the other HcWRKY family memebers were located in nucleus. Phylogenetic analysis showed that the 33 HcWRKY family members were divided into 4 groups. There was only HcWRKY member in Group Ⅱ and Group Ⅳ; while Groups Ⅰ and Ⅲ contained HcWRKY and AtWRKY proteins. qRT-PCR analysis indicated that 24 HcWRKY family members were induced by Cd stress. During the response to cadmium stress in kenaf, the expression levels of 22 HcWRKY family member genes were up-regulated, while the expression levels of 2 HcWRKY family member genes were down-regulated, which indicated that these HcWRKY genes improved greatly camdium tolerance of kenaf. These results provides basic information for further exploring the biological role of the HcWRKY gene family response to cadmium stress.
Key wordsKenaf    Cd stress    WRKY gene family
收稿日期: 2023-09-25     
中图分类号: S563.5
基金资助:湖南省教育厅重点项目(21A0422); 湖南省自然科学基金(2024JJ5290); 国家麻类产业技术体系(CARS-16-E5); 中国农业科学院科技创新工程(一年生麻类遗传改良)(ASTIP-IBFC03)
通讯作者: * luanmingbao@caas.cn   
引用本文:   
李辉, 陈安国, 唐慧娟, 栾明宝. 红麻基因组WRKY家族成员的鉴定及其在镉胁迫下的表达分析[J]. 农业生物技术学报, 2024, 32(10): 2243-2254.
LI Hui, CHEN An-Guo, TANG Hui-Juan, LUAN Ming-Bao. Identification of Kenaf (Hibiscus cannabinus) WRKY Family Member and Analysis of Their Expression Under Cadmium Stress. 农业生物技术学报, 2024, 32(10): 2243-2254.
链接本文:  
https://journal05.magtech.org.cn/Jwk_ny/CN/10.3969/j.issn.1674-7968.2024.10.004     或     https://journal05.magtech.org.cn/Jwk_ny/CN/Y2024/V32/I10/2243
 
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