Identification of CAPN11 Gene SNPs in White King Pigeons (Columba livia) and Their Impact on Slaughter Traits
XIANG Jin1, LONG Xia1, PANG Jin-Lan2, CHENG Min3, ZHANG Yi-Yu1,*, YANG A-Ming2,*
1 College of Animal Science/Key Laboratory of Genetic Breeding and Reproduction of Plateau and Mountain Animals/Key Laboratory of Animal Genetic Breeding and Reproduction of Guizhou Province, Guizhou University, Guiyang 550025, China; 2 Agriculture and Rural Bureau of Qianxi City, Qianxi 551500, China; 3 Guizhou Yuqian Agricultural Biotechnology Development Co., Ltd., Qianxi 551500, China
Abstract：White king pigeon (Columba livia) is an important economic poultry in China. To explore the relationship between calpain 11 (CAPN11) gene polymorphism and slaughtering traits is of great significance to the genetic improvement of slaughtering traits and the improvement of economic traits. In this study, SNP of CAPN11 gene of white king pigeon was screened by PCR direct sequencing method, and the relationship between SNP locus and slaughtering traits was analyzed. Through the identification of the SNPs of the CAPN11 gene of the white king pigeon, it was found that g.3641644C>T and g.3641746C>T 2 synonymous mutations were found at positions 636 and 738 in the exon 6 region of CAPN11 gene of the white king pigeon, all of which had 3 genotypes, the dominant genotypes and alleles were CT and C, 0.25<PIC<0.5. χ2 test showed that the genotypic distribution of the two SNPs loci were in accordance with Hardy-Weinberg equilibrium. Linkage disequilibrium analysis showed that D'>0.80 and r2>0.33 were not satisfied between the 2 SNPs, indicating that there was no strong linkage disequilibrium effect. Correlation analysis showed that g.3641644C>T locus had a significant effect on antemortem live weight (P<0.05). The effects of g.3641746C>T locus on pre-slaughter live weight, dressed weight, eviscerated weight, chest muscle weight and leg muscle weight of white king pigeons reached significant level (P<0.05). Diplotype analysis showed that 4 haplotypes and 10 diplotypes were detected at 2 SNPs loci in the experimental population. The antemortem live weight, dressed weight, eviscerated weight and leg muscle weight of individuals with haplotype H2H2 (CCTT) were significantly higher than those of the other 9 diplotypes, and the chest muscle weight of individuals with haplotype H2H2 (CCTT) was significantly higher than that of individuals with other diplotypes except H3H3 (TTCC) and H3H4 (TTCT). Through the analysis of the secondary structure of haplotype mRNA, it was found that haplotypes H1 (CC), H2 (CT) and H3 (TC) all caused changes in the mRNA secondary structure of CAPN11 gene, which might affect the efficiency of transcription and translation, and then affect the slaughtering performance. In summary, CAPN11 gene can be used as a potential candidate gene to improve molecular marker selection for slaughtering traits of white king pigeons. This study provides a theoretical basis for accelerating the breeding process of white king pigeons and reference for improving molecular marker breeding of slaughtering traits of white king pigeons.
向进, 龙霞, 庞金兰, 成敏, 张依裕, 杨阿明. 白羽王鸽CAPN11基因SNPs鉴定及其对屠宰性状的影响[J]. 农业生物技术学报, 2024, 32(10): 2381-2390.
XIANG Jin, LONG Xia, PANG Jin-Lan, CHENG Min, ZHANG Yi-Yu, YANG A-Ming. Identification of CAPN11 Gene SNPs in White King Pigeons (Columba livia) and Their Impact on Slaughter Traits. 农业生物技术学报, 2024, 32(10): 2381-2390.
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