Analysis of plekhm2 Gene Structure and Differential Expression in Taihang Chickens (Gallus domesticus)
DING Hong1, ZHANG Yin-Liang1, ZHOU Rong-Yan1,*, LI Yuan-Yuan1, LIU Hua-Ge2
1 College of Animal Science and Technology, Hebei Agricultural University, Baoding 071000, China; 2 Hebei Institute of Animal Husbandry and Veterinary Medicine, Baoding 071000, China
Abstract：Chicken salmonellosis is a highly infectious bacterial disease caused by Salmonella infection of chicken (Gallus domesticus). The pleckstrin homology and RUN domain containing M2 (plekhm2) gene acts as a target of the Salmonella effector protein SifA and plays a role in Salmonella infection by downregulating the recruitment of kinesin. The aim of this study was to analyze the sequence characteristics of the chicken plekhm2 gene and to investigate its tissue expression profile and the expression levels in the livers and spleens of Taihang chickens infected and uninfected with Salmonella. Differentially expressed genes were screened based on spleen transcriptome data from Salmonella-infected and uninfected chickens, online tools and software were used for bioinformatic analysis of plekhm2 gene, and qPCR was used to detect the expression levels of plekhm2 gene in different tissues of Taihang chickens, and a Taihang chicken Salmonella infection model was constructed to study the changes of its expression in the chicken liver and spleen. The results showed that the expression level of plekhm2 gene was elevated in the spleen-based transcriptome infected with Salmonella, which encoded 1 016 amino acids, with 2 motifs (RUN and PH), and the highest similarity of sequence with duck (Cairina moschata). Sequence comparison revealed a missense mutation site c.1013G>A at position 1 013 of the cDNA sequence of plekhm2 gene (p.Gly338Glu), A for Taihang chicken or G for Kashmir faverolla chicken; Tissue expression profiles showed that plekhm2 gene was expressed in 11 tissues of Taihang chickens, including heart, liver, spleen and lung. Compared with the uninfected group, the relative expression levels of plekhm2 gene in the liver (P<0.01) and spleen (P<0.05) of Taihang chickens were significantly higher after Salmonella infection. plekhm2 gene was expressed in several tissues of Taihang chickens, and Salmonella infection caused an increase in the expression level of plekhm2 gene. The present study provides a reference to reveal the function of plekhm2 gene in Salmonella infection.
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