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| Characterization of a Novel SNP of Porcine Adipocyte Determination and Differentiation Factor-1 Gene (ADD1) and Its Effects on Meat Quality |
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Abstract Adipocyte determination and dfferentiation factor-1 gene(ADD1), a gene associated with adipocyte differentiation in the research of human and rat, plays a role during adipocyte differentiation with PPARγ and C/EBP. In this paper, we cloned a part of the gene sequence from pig, screened it with PCR-SSCP (single strand conformation polymorphism), and used sequencing to identify mutation. The result indicated that a SNP (single nucleotide polymorphism) happened in the conservative domain of the ADD1 gene, which led to a different amino-acid sequence and a new site for proteinase. To verity the relationship between this mutation and meat quality, we chose 136 pigs as the experimental population, all of which were butchered to determine meat quality. The result of polymorphism analysis showed that a novel SNP was found in second exon. The polymorphism was disequilibrium in population and AA genotype was not found in it. The relation analysis showed that intramuscular protein, intramuscular fat and meat color had obviously difference among different ADD1 genotypes of pigs. The ADD1 polymorphism can apply to pig breeding for improving meat quality．
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Received: 13 March 2009
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