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| Selection of the Reference Genes of Real-time Quantitative PCR in the Gene Expression of Piglet Tissues |
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Abstract Abstract Selection of the best stable reference genes is of crucial importance for the accurate normalization of expression in real-time quantitative PCR. Taking the piglet tissues as example, the expressions of nine housekeeping geens beta-actin(ACTB), beta-2-microglobulin(B2M), glyceraldehyde-3-phosphate dehydrogenase(GAPDH), hydroxymethylbilane synthase(HMBS), hypoxanthine phosphoribosyltransferase 1(HPRT1), ribosomal protein L4(RPL4), succinate dehydrogenase complex, subunit A(SDHA), TATA box binding protein1(TBP1) and Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein and zeta polypeptide(YWHAZ) were detected using real-time quantitative PCR. And their expression stability were observed by analysis of geNorm program. Results showed HPRT1 and HMBS > RPL4 > TBP1> B2M > YWHAZ > SDHA > GAPDH > ACTB. As the best choice, HMBS can be used as normalizing expression of target gene in different piglet tissues, while RPL4 is good reference gene for high abundant transcripts.
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Received: 26 February 2009
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