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| Effect of G3909A Mutation in Pituitary Adenylate Cyclase-activating Polypeptide Gene(PACAP) on the Partial Growth Traits in Qinchuan Cattle |
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Abstract Abstract The polymorphisms of pituitary adenylate cyclase-activating polypeptide gene(PACAP) were detected in a 257 Qinchuan cattles(Bos tarurs) population by using PCR-SSCP and the assocation of genotype with partial growth traits was calculated by the least squire method(LSM). Result showed that a G/A mutation was found at the locus of 3909 bp, which result in 3 genotypes named AA,AB and BB respectively. Allele frequencies of A and B were 0.5992 and 0.4008, respectively. χ2 test showed that the locus was in Hardy-Weinberg equlibrium in the Qinchuan cattle population detected. Variance analysis showed: The body length of individuals with AA, AB and BB genetype were extremely significantly difference ( P<0.01); The wither height of individuals with AA and AB genotype was extremely significantly higher than those with BB (P<0.01), and those with AA genotype was significantly higher than those with AB ( P<0.05); The body weight of individuals with AA and AB genotype was extremely significantly higher than those with BB (P<0.01); The heart girth of individuals with AA genotype was extremely significantly higher than those with BB (P<0.01), those with AA genotype was significantly higher than those with AB genotype(P<0.05), and those with AB genotype was significantly higher than those with BB genotype( P<0.05); The heart width of individuals with AB genotype was significantly higher than those with BB ( P< 0.05); There were not significantly differences in the other growth traits among individuals with different genotypes. The locus can be a potential QTN affecting body length, wither height, body weight, heart girth and heart width or tightly linked with it. It will be a candidate moleculer marker for the Qinchuan cattle breeding.
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Received: 20 March 2009
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YANG Han, ZHANG Yang-Hai, WANG Min, KANG Yu-Xin, ZHU Hai-Jing, LAN Xian-Yong, QU Lei, YAN Hai-Long, PAN Chuan-Ying. Polymorphisms of 3'-UTR of POU1F1 Gene and Its Association with Growth Traits in Shaanbei White Cashmere Goats (Capra hircus)[J]. 农业生物技术学报, 2019, 27(7): 1224-1232. |
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