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| Study on the Production, Purification and Characteristics of the recombinant Chitinase from Rice |
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Abstract In a 7 L fermentor, the production conditions of engineering strain NB01 containing chitinase gene were investigated. By enhancing aerati0 and changing the agitation rate，the DO level was higher than 30%. The methanol feed rate was varied with the change of DO level in order to avoid the methanol concentration too higher to injure the cells. And the results showed that the maximum chitinase activity could reach 48.0795 U/mL at 84h. The fast purification of recombinant chitinase was conducted with Hollow fiber UF membrane and Sephadex G-100 and the SDS-PAGE analysis indicated that there is a single band with the right molecular weight corresponding to the chitinase on the gel. Furthermore, the recombinant enzyme is stable at a wide range of temperature and pH. Also, it is rather stable when stored at 4℃ for a long time.
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Received: 15 June 2007
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ZHAI Da-Cai, FANG Zhen, LV Cai-Yun, ZHANG Ming-Liang, LIU Shan, WU Meng, FANG Ru-Yue, ZHOU Xun, FANG Jian-Xin, BAI Xiao-Hui. Research on Antibacterial Activity of the Endophytic Bacillus subtilis HJ-2 from Polygonatum cyrtonema[J]. 农业生物技术学报, 2019, 27(9): 1664-1672. |
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