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| Research on Antibacterial Activity of the Endophytic Bacillus subtilis HJ-2 from Polygonatum cyrtonema |
| ZHAI Da-Cai1, FANG Zhen2, LV Cai-Yun1, ZHANG Ming-Liang1, LIU Shan1, WU Meng1, FANG Ru-Yue1, ZHOU Xun3, FANG Jian-Xin1, BAI Xiao-Hui1, * |
1 College of Life and Environment Science, Huangshan University, Huangshan 245041, China;
2 Chawang Forest Industry and Mining Farm of Qimen County Anhui Province , Huangshan 245600, China;
3 Analysis and Test Center, Huangshan University, Huangshan 245041, China |
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Abstract Endophytes, which can produce the same or similar metabolites as host plants, is an important resource for screening novel secondary metabolites. Thus, it has great significance to isolate and screen antagonistic endophytic bacterium from the wild Polygonatum cyrtonema and study the antibacterial activity of its metabolites. The endophytic bacterium HJ-2 (GenBank No. MK774703) isolated and screened from the rhizome of the wild P. cyrtonema was identified as Bacillus subtilis. In order to furtherly utilize the strain, different media were used for fermentation of the strain. The active substances were extracted from the bacterial cell of strain HJ-2 and its fermentation broth by methanol, ethanol, n-butanol, acetone and ethyl acetate, respectively. All the extracted products were used for physicochemical properties and antibacterial activity study. Our results showed that the antibacterial active substances produced by the strain were presented in the fermentation broth and had significant antibacterial effects against Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa. The fermentation products were further tested for acid and alkali resistance and heat resistance. The results showed that the products could resistance to high temperature of 65 ˚C, but had a poor performance of acid and alkali resistance. This study provides a theoretical basis for the exploration of endophytic bacteria resources of P. cyrtonema from Anhui province and the comprehensive development and utilization of strain HJ-2.
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Received: 10 February 2019
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Corresponding Authors:
bxh@hsu.edu.cn
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