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Developmental Changes of GnRH-I and ER-β mRNA
Expression in the Ovary of Prepubertal Shaoxing Ducks |
NI Ying-Dong ZHOU Yu-Chuan LU Li-Zhi CHEN Jie ZHAO Ru-Qian** |
| (Key Laboratory of Animal Physiology and Biochemistry, Nanjing Agricultural University, Nanjing 210095,China) |
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Abstract Abstract: Gonadotrophin-releasing hormone-1(GnRH-I )and estrogen receptor-β (ER-β ) mRNA expression in the ovary of Shaoxing ducks between 1 and 90 post hatching were measured by semi-quantitative RT-PCR using β-actin as an internal standard. The abundance of ovarian GnRH-I mRNA increased gradually from 1 to 90 d of age, 60-day-old ducks exhibited the highest level of ovarian GnRH-I mRNA. And the level of ovarian GnRH-I mRNA from 60 to 90 day was significantly higher than that of 1 to 30 d. However, the level of ER-β mRNA went up gradually from 1 d to 60 d, reaching a peak at 60 d, then went down to the level of 90 d. Ovarian expression of ER-β mRNA was significantly greater at 60 d than any of other age groups. The results indicated that ovarian GnRH-1 may be involved in regulating the growth of post-stage ovarian follicle, while ER-β mRNA may be participated in the earlier follicle development in Shaoxing ducks.
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Received: 01 January 1900
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Corresponding Authors:
ZHAO Ru-Qian
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