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Polymorphism of Jiangxi Indigenous Chicken Breeds and Its
Population Genetic Relationships Inferred by AFLP Analysis |
HU Xiao-Fen1，3** GAO Jun1** AI Hua-Shui1 DING Neng-Shui1 CHEN Cong-Ying1
GUO Yuan-Mei1 SHU Xi-Fan2 HUANG Lu-Sheng1 REN Jun1*** |
| (1.Key Laboratory for Animal Biotechnology， Jiangxi Province and Ministry of Agriculture, Jiangxi Agricultural University, Nanchang 330045, China; 2. Animal Husbandry and Veterinary Medicine Bureau, Agriculture Office of Jiangxi Province, Nanchang 330044, China; 3. College of Life Science, Hunan University of Science and Technology, Xiangtan 411201, China) |
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Abstract Abstract: A total of 15 pairs of AFLP primer combinations were used to detect genetic variation of pooled genomic DNA from 8 native chicken breeds and 1 cultivated chicken breed in Jiangxi Province as well as 1 exotic chicken breed, which included Nanchenghei, Yuganwuhei, Congrenma, Ningdusanhuang, Wanzaikanglehuang, Guangfeng Baierhuang, Dongxiang green-shell, Taihe silky fowl, Jingdehuang and Israel recessive white feather fowl. The genetic similarity coefficients of 10 chicken breeds were calculated from AFLP data, the highest similarity coefficient of 0.7790 existed between Yuganwuhei and Nanchenghei, followed by that of 0.6726 between Guangfeng Baierhuang and Jingdehuang, while the lowest similarity coefficient of 0.2801 was revealed between Israel recessive white feather fowl and Congrenma. UPGMA (unweighted pair group method with arithmetic mean) cluster analysis was also performed by the program of PHYLIP. Eight Jiangxi indigenous chicken breeds and the cultivated breed (Jingdehuang) were grouped into one branch, in which Yuganwuhei and Nanchenghei clustered together, and Guangfeng Baierhuang and Jingdehuang were grouped closely, while exotic chicken breed (Israel recessive white feather fowl) formed another branch. The results indicated that Jiangxi indigenous chicken breeds and exotic chicken breed had distant genetic relationship, the closest geneticrelationship was observed between Nanchenghei and Yuganwuhei, which was consistent with the previous result by RAPD analysis. The two chicken breeds were therefore suggested to be combined for conservation. Guangfeng Baierhuang and Jingdehuang showed intimate relationship, the two breeds was closer than any other pair of breeds, which was also consistent with the RAPD result and the breeding history of Jingdehuang chicken breed using Guangfeng Baierhuang as one of founder animals.
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Received: 01 January 1900
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Corresponding Authors:
REN Jun
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