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| Optimization on Process of Transgenic Mice Production |
| ZHENG Min2 WANG Li-Li1 WANG Mei-Li2 NIU Hui-Ling2 DAI Yun-Ping1* |
(1. State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing 100094, China;
2. Gentitan Biotechnology Co. LTD, Beijing 100094, China) |
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Abstract Abstract: The microinjection and implantation conditions were optimized by Kunmin mice as donor and receipt in the production of transgenic mice. Simultaneously the DNA concentrations in different size and standard of receipt selecting were redressed. Eventually the positive rates of transgenic mice were improved from 10% to 20%; the optimum suitability of DNA concentration was 2~3 μg/mL when size of DNA fragment exceed 10 kb.
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Received: 01 January 1900
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Corresponding Authors:
DAI Yun-Ping
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