Fine Mapping of Inflorescence Pruning Node Gene in Tomato (Solanum lycopersicum) Based on BSA-seq Technique
SHI Hai-Lin1, ZHANG Dan-Dan1, GAO Qian4, YOU Yuan-Yuan1, SHU Jin-Shuai5, WANG Shuai1,2,3*, MAO Xiu-Jie1,2,3
1 College of Horticultural Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao 066004, China; 2 Hebei Key Laboratory of Characteristic Horticultural Germplasm Mining and Innovative Utilization, Qinhuangdao 066004, China; 3 Hebei Higher Institute Application Technology Research and Development Center of Horticultural Plant Biological Breeding, Qinhuangdao 066004, China; 4 Changli Institute of Pomology, Hebei Academy of Agricultural and Forestry Sciences, Changli 066000, China; 5 Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Abstract The number of main stem flowers affects the yield of self-pruning tomato (Solanum lycopersicum). In order to locate the genes associated with inflorescence pruning node, a fine mapping was carried out for laying a foundation for gene cloning and functional analysis and providing evidences for the analysis of regulation mechanism. In this study, one F2 population was constructed from the cross between AXF (low nodal self-pruning tomato strain) and GXF (nodal self-pruning tomato strain) , and linkage analysis and gene localization were performed by bulked segregant analysis sequencing (BSA-seq) and molecular marker. The results showed that the associated region from 47.56~47.59 Mb was located on chromosome 2 with the size of 25 497 bp, containing 3 candidate genes (thiosulfate sulfurtransferase 18 (TST18) (GenBank No. XM_004232452.4), MLO-like protein 4 (MLO4) (GenBank No. XM_019212390.2) and 26S proteasome non- ATPase regulatory subunit 4 (PSMD4) (GenBank No. XM_010318126.3)). 118 InDel (insertion-deletion)/ CAPS (cleaved amplified polymorphism sequences)/dCAPS (derived CAPS) primers were designed for fine mapping and a dCAPS14 molecular maker closely linked with candidate gene PSMD4 was finally delimited by further analysis. qRT-PCR analysis showed that the relative expression of the candidate gene was significantly different between AXF and GXF. The PSMD4 was involved in ubiquitin-proteasome system mediated protein degradation and served as key candidate gene. PSMD4 might play an important role in the controlling of inflorescence pruning node. These results had a significance in marker-assisted self-pruning tomato breeding for ideal architecture with easy cultivation.
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