基于BSA-seq技术对番茄封顶花序节位基因的精细定位

doi:10.3969/j.issn.1674-7968.2023.07.003

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Abstract The number of main stem flowers affects the yield of self-pruning tomato (Solanum lycopersicum). In order to locate the genes associated with inflorescence pruning node, a fine mapping was carried out for laying a foundation for gene cloning and functional analysis and providing evidences for the analysis of regulation mechanism. In this study, one F2 population was constructed from the cross between AXF (low nodal self-pruning tomato strain) and GXF (nodal self-pruning tomato strain) , and linkage analysis and gene localization were performed by bulked segregant analysis sequencing (BSA-seq) and molecular marker. The results showed that the associated region from 47.56~47.59 Mb was located on chromosome 2 with the size of 25 497 bp, containing 3 candidate genes (thiosulfate sulfurtransferase 18 (TST18) (GenBank No. XM_004232452.4), MLO-like protein 4 (MLO4) (GenBank No. XM_019212390.2) and 26S proteasome non- ATPase regulatory subunit 4 (PSMD4) (GenBank No. XM_010318126.3)). 118 InDel (insertion-deletion)/ CAPS (cleaved amplified polymorphism sequences)/dCAPS (derived CAPS) primers were designed for fine mapping and a dCAPS14 molecular maker closely linked with candidate gene PSMD4 was finally delimited by further analysis. qRT-PCR analysis showed that the relative expression of the candidate gene was significantly different between AXF and GXF. The PSMD4 was involved in ubiquitin-proteasome system mediated protein degradation and served as key candidate gene. PSMD4 might play an important role in the controlling of inflorescence pruning node. These results had a significance in marker-assisted self-pruning tomato breeding for ideal architecture with easy cultivation.

Key words： Self-pruning tomato Bulked segregant analysis sequencing (BSA-seq) Molecular marker Gene localization

Received: 22 January 2023

Corresponding Authors: *wangshuai101og@126.com

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http://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2023.07.003 OR http://journal05.magtech.org.cn/Jwk_ny/EN/Y2023/V31/I7/1357

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