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| Phenotypic Analysis and Gene Fine Mapping of A Semi-dwarf Multi-tiller Mutant in Rice (Oryza sativa) |
| CHEN Li-Ping1,2,*, JIANG Jia-Huan1,2,*, ZHU Yong-Sheng1,2, ZHENG Yan-Mei1,2, LIN Qiang1,2, XIE Hong-Guang1,2, WANG Ying-Heng1,2, CAI Qiu-Hua1,2, XIE Hua-An1,2,**, ZHANG Jian-Fu1,2,** |
1 Rice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350019, China;
2 Key Laboratory of Germplasm Innovation and Molecular Breeding of Hybrid Rice for South China, Ministry of Agriculture and Rural, P.R. China/ Fuzhou Branch, National Rice Improvement Center of China/Fujian Engineering Laboratory of Crop Molecular Breeding/ Fujian Key Laboratory of Rice Molecular Breeding/Incubator of National Key Laboratory of Fujian Germplasm Innovation and Molecular Breeding Between Fujian and Ministry of Sciences & Technology/Base of South-China, National Key Laboratory of Hybrid Rice/National Rice Engineering Laboratory of China, Fuzhou 350003, China |
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Abstract Plant height and tiller number are important agronomic traits affecting rice (Oryza sativa) yield. Appropriate plant height and tiller number are beneficial to increase rice yield. In this study, a semi-dwarf mutant with multi-tiller, sd-fh7185 (semi-dwarf from Fuhui 7185), was screened from the mutant library of indica restorer line 'Fuhui 7185' (FH7185) with chemical mutating of 0.1% ethylmethylsulfone (EMS). The investigated and analyzed results showed that the plant height of sd-fh7185 decreased significantly at seedling stage, tillering stage and maturity stage compared with wild type FH7185 (P<0.01), the decrease of plant height was mainly caused by the shortening of panicle and the first internode, and sd-fh7185 was controlled by a single recessive nuclear gene located on chromosome 3 (Chr. 3) in rice. Map-based cloning results indicated that the target gene, sd-fh7185, was finally fine-mapped within a physical interval of about 120 kb between InDel markers ID73 and GH25 on Chr. 3. The target region contained 14 predicted functional genes, one of which was a documented dwarf multi-tiller gene tensinte branched 1 (OsTB1)/fine cuml 1 (FC1) (MSU-RGAP locus LOC_Os03g49880) in rice. The sequencing results revealed a single base substitution from C (FH7185, wild type, WT) to T (sd-fh7185) occurred at position 439 bp of the exon of LOC_Os03g49880 in sd-fh7185, which resulted in a premature termination, and the length of encoded product was shortened from 388 amino acids (WT) to 146 amino acids (sd-fh7185) with changed function. Summarily, sd-fh7185 was a newly identified allele of OsTB1/FC1 differed from previously reported fc1-1 and fc1-2, which enriched the semi-dwarf and multi-tillering gene resources in rice, and the results provide informative references for both the dissection of molecular mechanism and rice morphology breeding in rice.
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Received: 22 December 2021
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Corresponding Authors:
**jianfzhang@163.com; huaanxie@163.com
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| About author:: * These authors contributed equally to this work |
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