Cloning and Function Study of CaDDB1 Gene in Pepper (Capsicum annuum)
SHAN Qing-Yun, YUAN Qiao-Ling, GUO Si-Si, XIONG Cheng, LIU Feng*, ZOU Xue-Xiao*
Engineering Research Center for Horticultural Crop Germplasm Creation and New Variety Breeding, Ministry of Education/Key Laboratory for Vegetable Biology of Hunan Province, College of Horticulture, Hunan Agricultural University, Changsha 410128, China
Abstract Pepper (Capsicum annuum) is an annual or limited perennial herb of the Solanaceae and Capsicum genus. The damaged DNA binding protein 1 (DDB1) gene has been reported in tomato (Solanum lycopersicum), Arabidopsis thaliana and other model plants, but its function in pepper is still unclear. In this study, tomato DDB1 homologous gene was cloned from pepper and named CaDDB1 (GenBank No. XM_016701551). CaDDB1 gene silencing plants were obtained by using virus-induced gene silencing (VIGS) technique, and the biological functions of CaDDB1 were preliminarily identified through observing their phenotypes. The results showed that pepper plants were extremely significantly dwarfed after CaDDB1 gene silencing (P<0.01). Simultaneously, anthocyanin content in the 3rd to 6th true leaves were extremely significantly increased (P<0.01), indicated that CaDDB1 gene affected the growth and development of pepper and played a negative role in the regulation of anthocyanin synthesis. The results of this study provide some theoretical guidance for the growth and development regulation and variety improvement of pepper.
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