1 College of Life Sciences/Institute of Agro-bioengineering/Key Laboratory of Plant Resources Conservation and Germplasm Innovationin in Mountainous Region (Ministry of Education), Guizhou University, Guiyang 550025, China; 2 College of Tea Sciences, Guizhou University, Guiyang 550025, China; 3 Guizhou Academy of Agricultural Sciences, Guiyang 550006, China
Abstract Tea plants (Camellia sinensis), as one of the important woody economic plants, are vulnerable to low temperature and freezing injury, which seriously affecting the yield and quality of tea. In order to further clarify the molecular mechanism on cold response of tea plants, in this study, based on the cold treatment transcriptome library of 2 Guizhou cultivars, Qiancha 1 (QC1) and Qianmei 601 (QM601), a cold-upregulated differentially expressed mitogen-activated protein kinase (MAPK) gene (transcriptome library number: CSS0027011) was isolated and cloned, and named as CsMAPK15 (GenBank No. ON39909). The open reading frame of CsMAPK15 contains 1 701 nucleotides encoding a protein of 566 amino acids. Motif analysis revealed that CsMAPK15 had 3 conserved motifs. Phylogenetic analysis showed that CsMAPK15 shared high sequence similarity with homologs from other species. It shared 79.17% identity with AtMAPK16 (AT5G19010) from Arabidopsis and 80.51 % similarity with OsMAPK15 (LOC_Os07g47490) from rice (Oryza sativa). The tissue pattern analysis revealed that expression level of CsMAPK15 was highest in roots. Promoter analysis showed that the CsMAPK15 promoter had several cis-acting elements, including stress responsive element (STRE) and salicylic acid (SA) responsive element (TCA element). qPCR confirmed that CsMAPK15 could response to cold signal and SA. Furthermore, the transgenic rice plants which overexpressed CsMAPK15 were successfully obtained by callus transformation. The preliminary results showed that there was no significant change in plant growth and development between transgenic plants and wild-type plants. This study provides research materials and theoretical basis for further clarifying the role of CsMAPK15 in plant cold resistance regulation．
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