小麦Whirly基因家族的鉴定及表达分析

doi:10.3969/j.issn.1674-7968.2023.02.001

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Abstract Whirly family is a kind of plant-specific transcription factors, which have extensive and complex biological functions in both the nucleus and organelles. Because of the rotating appearance of its quaternary structure, it is called Whirly protein, and the corresponding gene is named Whirly gene. This family plays an important role in plant growth and stress response. In order to explore the function of wheat (Triticum aestivum) Whirly family, the basic characteristics of TaWHY gene family members were analyzed by bioinformatics method, and their responses to various stresses were verified by qPCR. The results showed that there were 6 members in TaWHY family, named TaWHY1-A, TaWHY1-A-1, TaWHY1-D, TaWHY2-A, TaWHY2-B and TaWHY2-D, respectively, which were distributed on 6 chromosomes of wheat, and all the member sequences contained introns. The results showed that TaWHYs protein instability index was 30~60, and the average coefficient of hydrophilicity was less than 0, which indicated that wheat Whirly protein was stabled in nature and belongs to hydrophilic protein. TaWHY protein was distributed in mitochondria, chloroplast, nucleus and cytoplasm. The secondary structure of TaWHY protein was mainly alpha helix and random curl, and there were 2 three-dimensional structure models. Transcriptional data analysis showed that TaWHY gene showed different tissue expression patterns and was regulated by different abiotic stresses. The results of qPCR showed that the relative expression of TaWHY1-A gene was significantly up-regulated under low temperature stress conditions(P<0.05), which could respond to low temperature stress. The results of this study can provide reference for further functional verification and mechanism analysis of TaWHY.

Key words： Wheat Whirly gene family Bioinformatics analysis Abiotic stress Expression analysis

Received: 16 March 2022

ZTFLH:

S330

Corresponding Authors: *wamail_wang@163.com;tangyimiao@126.com

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	SUN Yue
	YANG Hui-Yu
	ZHU Wen-Gen
	WANG Xiao-Yan
	TANG Yi-Miao

Cite this article:

SUN Yue,YANG Hui-Yu,ZHU Wen-Gen, et al. Identification and Expression Analysis of Wheat (Triticum aestivum) Whirly Gene Family[J]. 农业生物技术学报, 2023, 31(2): 223-231.

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http://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2023.02.001 OR http://journal05.magtech.org.cn/Jwk_ny/EN/Y2023/V31/I2/223

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