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Identification of MYB Gene Family and Its Expression Pattern Analysis Under Different Light Quality in Spine Grape (Vitis davidii) |
QUE Qiu-Xia1,2, LAI Gong-Ti2, PAN Ruo1,2, ZHANG Jing1,2, HE Li-Yuan1,2, LAI Pu-Fu2, WANG Qi2, GAO Hui-Ying2, CHEN Gui-Xin1,*, LAI Cheng-Chun2,* |
1 College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China; 2 Institute of Agricultural Engineering Technology, Fujian Academy of Agricultural Sciences, Fuzhou 350003, China |
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Abstract MYB transcription factor is an important regulatory factor in plant growth and secondary metabolism, and plays key roles in anthocyanin biosynthesis. In this research, 9 VdMYB genes were identified and cloned from Vitis davidii callus based on previous transcriptome data. The MYB gene structure and function were analyzed and predicated by bioinformatic softwares, and their expression patterns were analyzed under different light quality. The results showed that most MYB genes included 3 exons and 2 introns; the number of amino acid residues of VdMYBs were 216~335, the relative molecular mass were 24.09~36.17 kD, and the isoelectric point were 5.42~9.58. The phylogenetic tree analysis indicated that the 9 VdMYB proteins were divided into 6 groups, and were predicted to be located in cytoplasm, mitochondria and nucleus, respectively. Analysis of promoter cis-acting elements showed that a large number of light-response and bio/abio-response elements were predicted in the 9 VdMYB promoters. The qRT-PCR analysis showed that light quality significantly affected the expression of VdMYBs, their expression pattern corresponded to the structural characteristics under different light quality. Among 9 VdMYB genes, VdMYB31/VdMYBB1 and VdMYB4A might play as positive and negative regulators involved in anthocyanin biosynthesis.This study provides theoretical reference for further elucidating the light-response molecular mechanism of MYB family genes involved in the regulatory pathways of anthocyanins in Vitis davidii.
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Received: 23 May 2022
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Corresponding Authors:
*lccisland@163.com;guixinchen@126.com
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[1] 焦晓博, 罗尧幸, 赵伟, 等. 2018. 葡萄MYB基因家族及其对花器官性别分化调控的分析[J]. 山西农业大学学报(自然科学版), 38(05): 23-32, 39. (Jiao X B. Luo Y X. Zhao W. et al. 2018. Analysis of grape MYB gene family and its regulation on sex differentiation of flower organs[J]. Journal of Shanxi Agricultural University (Natural Science Edition, 38(05): 23-32, 39.) [2] 赖呈纯, 范丽华, 黄贤贵, 等. 2014. 刺葡萄幼胚愈伤组织诱导及其高产原花青素细胞系筛选[J]. 植物生理学报, 50(11): 1683-1691. (Liu C C, Fan L H, Huang X G, et al.2014. Callus induction in brier grape (Vitis davidii Foëx) from immature embryos and screening of cell lines with high-production of oligomeric proanthocyanidins[J]. Plant Physiology Journal, 50(11): 1683-1691.) [3] 潘红, 赖呈纯, 张静, 等. 2019. 不同光质条件下刺葡萄红色愈伤组织的RT-qPCR内参基因筛选[J]. 应用与环境生物学报, 25(06): 1407-1413. (Pan H, Lai C C, Zhang J, et al.2019. Selection of reference genes for RT-qPCR from the red callus of Vitis davidii (Rom. Caill.) Foëx under different light qualities[J]. Chinese Journal of Applied & Environmental Biology, 25(6): 1407-1413.) [4] 吴瑞, 林定, 罗栋, 等. 2022. 番红花R2R3-MYB转录因子的鉴定与时空表达分析[J]. 农业生物技术学报, 30(03): 457-472. (Wu R, Lin D, Luo D, et al.2022. Identification and spatiotemporal expression analysis of R2R3-MYB transcription factor in Crocus sativus[J]. Journal of Agricultural Biotechnology, 30(3): 457-472.) [5] 左然, 徐美玲, 柴国华, 等. 2012. 植物MYB转录因子功能及调控机制研究进展[J]. 生命科学, 24(10): 1133-1140. (Zuo R, Xu M L, Chai G H, et al.2022. Function and regulation mechanism of plant MYB transcription factors[J]. Chinese Bulletin of Life Sciences, 24(10): 1133-1140.) [6] 钟秀来, 罗庆, 张德军, 等. 2021. 不同光质条件下紫色芹菜花青素响应机理分析[J]. 分子植物育种, 19(19): 6529-6535. (Zhong X L, Luo Q, Zhang D J, et al.2021. Response mechanism analysis of anthocyanin in purple celery under light quality condition[J]. Molecular Plant Breeding, 19(19): 6529-6535.) [7] Agarwal T, Grotewold E, Doseff A L, et al.2016. MYB31/MYB42 syntelogs exhibit divergent regulation of phenylpropanoid genes in maize, sorghum and rice[J]. Scientific Reports, 6(1): 1-17. [8] Anwar M, Wang G, Wu J, et al.2018. Ectopic overexpression of a novel R2R3-MYB, NtMYB2 from Chinese narcissus represses anthocyanin biosynthesis in tobacco[J]. Molecules, 23(4): 781-798. [9] Bendokas V, Skemiene K, Trumbeckaite S, et al.2020. Anthocyanins: From plant pigments to health benefits at mitochondrial level[J]. Critical Reviews in Food Science and Nutrition, 60(19): 3352-3365. [10] Bian Z H, Yang Q C, Liu W K, et al.2015. Effects of light quality on the accumulation of phytochemicals in vegetables produced in controlled environments: A review[J]. Journal of the Science of Food and Agriculture, 95(5): 869-877. [11] Chen Y H, Yang X Y, He K, et al.2006. The MYB transcription factor superfamily of Arabidopsis: Expression analysis and phylogenetic comparison with the rice MYB family[J]. Plant Molecular Biology, 60(1): 107-124. [12] De Wit M, Galvao V C, Fankhauser C, et al.2016. Light-mediated hormonal regulation of plant growth and development[J]. Annual Review of Plant Biology, 67: 513-537. [13] Efferth T.2019. Biotechnology applications of plant callus cultures[J]. Engineering, 5(1): 50-59. [14] Franklin K A.2009. Light and temperature signal crosstalk in plant development[J]. Current Opinion in Plant Biology, 12(1): 63-68. [15] Guan L, Dai Z W, Wu B H, et al.2016. Anthocyanin biosynthesis is differentially regulated by light in the skin and flesh of white-fleshed and teinturier grape berries[J]. Planta, 243(1): 23-41. [16] Kobayashi S, Goto-Yamamoto N, Hirochika H, et al.2004. Retrotransposon-induced mutations in grape skin color[J]. Science, 304(5673): 982-982. [17] Landi M, Tattini M, Could K S.2015. Multiple functional roles of anthocyanins in plant-environment interactions[J]. Environmental and Experimental Botany, 119: 4-17. [18] Liang N N, Pan Q H, He F, et al.2013. Phenolic profiles of Vitis davidii and Vitis quinquangularis species native to China[J]. Journal of Agricultural and Food Chemistry, 61(25): 6016-6027. [19] Matus J T, Aquea F, Arce-Johnson P, et al.2008. Analysis of the grape MYB R2R3 subfamily reveals expanded wine quality-related clades and conserved gene structure organization across Vitis and Arabidopsis genomes[J]. BMC Plant Biology, 8(1): 1-15. [20] Mmadi M A, Dossa K, Wang L, et al.2017. Functional characterization of the versatile MYB gene family uncovered their important roles in plant development and responses to drought and waterlogging in sesame[J]. Genes, 8(12): 362-379. [21] Mushtaq M A, Pan Q, Chen D, et al.2016. Comparative leaves transcriptome analysis emphasizing on accumulation of anthocyanins in Brassica: Molecular regulation and potential interaction with photosynthesis[J]. Frontiers in Plant Science, 7: 311. [22] Naing A H, Kim C K.2018. Roles of R2R3-MYB transcription factors in transcriptional regulation of anthocyanin biosynthesis in horticultural plants[J]. Plant Molecular Biology, 98(1): 1-18. [23] Ni J B, Apekshika T, Premathilake, et al.2021. Ethylene-activated PpERF105 induces the expression of the repressor-type R2R3-MYB gene PpMYB140 to inhibit anthocyanin biosynthesis in red pear fruit[J]. The Plant Journal, 105(1): 167-181. [24] Niu S Y, Hao F G, Mo H Z, et al.2017. Examination of molecular mechanism for the color mutation in Chinese wild grapevine (Vitis davidii)[J]. Acta Physiologiae Plantarum, 39(8): 171-181. [25] Niu T Q, Gao Z D, Zhang P F, et al.2016. MYBA2 gene involved in anthocyanin and flavonol biosynthesis pathways in grapevine[J]. Genetics and Molecular Research, 15(4): 8922-8933. [26] Ren D Y, Rao Y C, Yu H P, et al.2020. MORE FLORET1 encodes a MYB transcription factor that regulates spikelet development in rice[J]. Plant Physiology, 184(1): 251-265. [27] Samkumar A, Jones D, Karppinen K, et al.2021. Red and blue light treatments of ripening bilberry fruits reveal differences in signalling through abscisic acid‐regulated anthocyanin biosynthesis[J]. Plant, Cell, and Environment, 44(10): 3227-3245. [28] Shi L Y, Cao S F, Chen W, et al.2014. Blue light induced anthocyanin accumulation and expression of associated genes in Chinese bayberry fruit[J]. Scientia Horticulturae, 179: 98-102. [29] Stracke R, Werber M, Weisshaar B, et al.2001. The R2R3-MYB gene family in Arabidopsis thaliana[J]. Current Opinion in Plant Biology, 4(5): 447-456. [30] Tao R Y, Bai S L, Ni J B, et al.2018. The blue light signal transduction pathway is involved in anthocyanin accumulation in 'Red Zaosu' pear[J]. Planta, 248(1): 37-48. [31] Villegas D, Handford M, Alcalde J A, et al.2016. Exogenous application of pectin-derived oligosaccharides to grape berries modifies anthocyanin accumulation, composition and gene expression[J]. Plant Physiology and Biochemistry, 104: 125-133. [32] Wang L, Luo Z S, Yang M Y, et al.2022. The action of RED light: Specific elevation of pelargonidin-based anthocyanin through ABA-related pathway in strawberry[J]. Postharvest Biology and Technology, 186: 111835-111842. [33] Wang X Y, Tian L, Feng S J, et al.2022. Identifying potential flavonoid biosynthesis regulator in Zanthoxylum bungeanum Maxim. by genome-wide characterization of the MYB transcription factor gene family[J]. Journal of Integrative Agriculture, 21(7): 1997-2018. [34] Wilkins O, Nahal H, Foong J, et al.2009. Expansion and diversification of the Populus R2R3-MYB family of transcription factors[J]. Plant Physiology, 149(2): 981-993. [35] Xie S, Qiao X L, Chen H W, et al.2019. Coordinated regulation of grape berry flesh color by transcriptional activators and repressors[J]. Journal of Agricultural and Food Chemistry, 67(42): 11815-11824. [36] Xu Z S, Yang Q Q, Feng K, et al.2020. DcMYB113, a root-specific R2R3-MYB, conditions anthocyanin biosynthesis and modification in carrot[J]. Plant Biotechnology Journal, 18(7): 1585-1597. [37] Yang T, Ma H Y, Li Y, et al.2021. Apple MPK4 mediates phosphorylation of MYB1 to enhance light-induced anthocyanin accumulation[J]. The Plant Journal, 106(6): 1728-1745. [38] Yu M, Man Y P, Wang Y C.2019. Light- and temperature-induced expression of an R2R3-MYB gene regulates anthocyanin biosynthesis in red-fleshed kiwifruit[J]. International Journal of Molecular Sciences, 20(20): 5228. [39] Zhang C Y, Long Y, Feng J, et al.2007. Transcriptional regulation of plant genes and its significance in biology[J]. Hereditas, 29(7): 793-799. [40] Zhang P F, Dong T M, Wen H Y, et al.2019. Knockdown of VvMYBA1 via virus-induced gene silencing decreases anthocyanin biosynthesis in grape berries[J]. Canadian Journal of Plant Science, 100(2): 175-184. [41] Zhao W J, Tang D B,Yuan E, et al.2020. Inducement and cultivation of novel red Cyclocarya paliurus callus and its unique morphological and metabolic characteristics[J]. Industrial Crops and Products, 147: 112266. [42] Zhou Y, Singh B R.2002. Red light stimulates flowering and anthocyanin biosynthesis in American cranberry[J]. Plant Growth Regulation, 38(2): 165-171. [43] Zhu N, Cheng S F, Liu X Y, et al.2015. The R2R3-type MYB gene OsMYB91 has a function in coordinating plant growth and salt stress tolerance in rice[J]. Plant Science, 236: 146-156. |
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