木薯ABA受体<em>MePYL4a</em>在拟南芥中过表达增强其耐旱性

doi:10.3969/j.issn.1674-7968.2022.12.003

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Abstract Abscisic acid (ABA) and its receptor pyrabactin resistance like (PYL) play an important role in plant resistance to drought stress. In order to explore the drought resistance function of the MePYL4a (GenBank No. XM_021766535) gene of cassava (Manihot esculenta), the MePYL4a gene was cloned from the cassava variety 'SC8', and its bioinformatics analysis was carried out. The subcellular localization was carried out using the Agrobacterium tumefaciens mediated transient transformation method; The expression of MePYL4a gene under drought stress was detected by qPCR; MePYL4a overexpression vector was constructed and transformed into Arabidopsis thaliana for drought resistance analysis of A. thaliana transgenic plants. The results showed that MePYL4a was an unstable protein, and the subcellular localization characteristics of MePYL4a protein showed that it was located in the nucleus and cytoplasm; qPCR analysis showed that MePYL4a gene expression level was significantly induced by drought stress, reached the highest level in 1 h, and then slowly decreased. After drought treatment, the phenotype observation showed that MePYL4a could improve the drought tolerance of A. thaliana seedlings under drought stress. The average biomass of over expressed plants was 1.5 times that of wide type (WT). The expression of key rate limiting enzyme gene 9-cis-epoxycarotenoid dioxygenase (NCED3), and drought stress response index gene responsive to dehydration (RD29A) for ABA synthesis was significantly higher than that of WT and Atpyl4 mutant plant. And the activity of antioxidant enzymes was about twice higher than that of WT, indicating that the ability of over expressed plants of MePYL4a to remove active oxygen was stronger than that of WT and Atpyl4 plant. Under drought stress, it could accumulate more biomass and enhance the drought resistance of A. thaliana. In conclusion, MePYL4a overexpression reduced the accumulation of reactive oxygen species in A. thaliana under drought stress and induced higher expression of stress resistance genes, which enhanced drought resistance of A. thaliana. This study provides a reference for MePYL4a to participate in cassava stress resistance.

Key words： Cassava M. esculenta pyrabactin resistance 1-like 4a (MePYL4a) Drought stress Gene expression Physiological and biochemical indexes

Received: 27 April 2022

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Corresponding Authors: * wangwenquan@itbb.org.cn; chenxin@itbb.org.cn

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	ZHAO Dong-Li
	BAO Ru-Xue
	LI Meng-Tao
	CHEN Xin
	WANG Wen-Quan

Cite this article:

ZHAO Dong-Li,BAO Ru-Xue,LI Meng-Tao, et al. Overexpression of Cassava (Manihot esculenta) ABA Receptor MePYL4a in Arabidopsis thaliana Enhances Its Drought Tolerance[J]. 农业生物技术学报, 2022, 30(12): 2290-2300.

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http://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2022.12.003 OR http://journal05.magtech.org.cn/Jwk_ny/EN/Y2022/V30/I12/2290

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