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| Expression Analysis of Molybdenum Cofactor Sulfurase Gene in Grape (Vitis vinfera) in Response to Light Stress |
| ZHANG Min1,2, DONG Yi-Ling2, XIAO Xu-Teng2, CHEN Tian-Chi2, SHEN Le-Yi2, JIA Yong-Hong2, WU Yue-Yan2,* |
1 College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China;
2 College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo 315100, China |
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Abstract Molybdenum cofactor sulfurase (MCSU or LOS5) of plants plays an important role in resisting non-biological stress resistance. At present, few studies reported on the regulation of abscisic acid (ABA) synthesis by VvMCSU gene in response to light stress in grape (Vitis vinfera). VvMCSU gene was cloned by PCR with a full length of coding region, then predicted the gene function from the encoded amino acid sequence by bioinformatics. Subsequently, the expression profiles of VvMCSU in response to light shading stress in leaves were analyzed by qPCR in combination with their leaf photosynthetic characteristics. The results were shown below: Under heavy shading, the maximum photosynthetic quantum yield (Fv/Fm) and non-photochemical quenching parameter (qN) increased by 4.32 % and 28.13 % respectively, and the leaf width and the content of ABA significantly increased by 10.16% and by 1.50 times compared with the control group. The coding region of VvMCSU gene was 2 478 bp, which encodes 825 amino acids, and its promoter region had several cis elements in response to abiotic stress and hormone. VvMCSU was a hydrophilic protein with MCSU structure, which interacted with proteins related to ABA synthesis, and belongs to the same evolutionary branch as camellia (Camelia sinensis). The expression of VvMCSU was up-regulated under 60 % light shading, which was 2.77 times higher than that of the control group 0 % shading or under natural light; and 4.01 times higher than that of 80% severe shading. The expression of VvMCSU gene was positively correlated with ABA content and ratios Fv/Fm (P<0.05). In this study, chlorophyll fluorescence and ABA content were used to analyze the expression of VvMCSU under different light conditions. The results suggested that VvMCSU was an important regulatory gene involved in ABA metabolism in response to light shading stress of grape. This study provides a theoretical basis for viniculture studies to improve grape shading tolerance.
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Received: 23 March 2022
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Corresponding Authors:
* wyynb2009@163.com
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