Complementary Expression Vector Construction and Drought Resistance Analysis of GhGGB Gene in Cotton (Gossypium hirsutum)
HU Zi-Yao1,*, AERZUGULI·Ta-Shi2,*, LEI Jian-Feng2, DAI Pei-Hong1, LIU Jian-Fei1, DENG Jia-Hui1, LIU Chao1, LIU Xiao-Dong1, LI Yue1,**
1 College of Life Sciences, Xinjiang Agricultural University, Urumqi 830052, China; 2 College of Agronomy, Xinjiang Agricultural University, Urumqi 830052, China
AbstractGGB (geranyl geranyltransferase beta) gene encodes type Ⅰ protein geranyltransferase, which is involved in protein isoprene modification and plays an important regulatory role in plant response to stress. In order to explore the biological function of GhGGB gene in cotton (Gossypium hirsutum), the plant complementary expression vector pBI101-AtGGBP-GhGGB-AtGGBT was constructed and transformed into ggb mutant of Arabidopsis thaliana, and GhGGB transgenic lines were screened. Phenotypic, physiological and biochemical indexes of GhGGB transgenic lines, ggb mutant and wild type Arabidopsis Col-0 under natural drought stress were detected. The results showed that with wild-type Arabidopsis Col-0 as the control, the sensitivity of transgenic GhGGB plants to drought stress was significantly enhanced, while the ggb mutant plants showed stronger drought resistance. The determination results of physiological indexes of each component were consistent with the phenotype, indicating that GhGGB gene complemented the functional deficiency of ggb mutant in Arabidopsis and reduced drought tolerance. The results of this study preliminarily showed that GhGGB was a negative regulatory gene for drought resistance, which provides a new gene selection for cotton drought-resistant germplasm cultivation.
HU Zi-Yao,AERZUGULI·,Ta-Shi, et al. Complementary Expression Vector Construction and Drought Resistance Analysis of GhGGB Gene in Cotton (Gossypium hirsutum)[J]. 农业生物技术学报, 2022, 30(9): 1687-1697.
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