木豆MYB-related亚家族基因鉴定及干旱胁迫下<em>CcMYB</em>-<em>R48</em>的功能解析

doi:10.3969/j.issn.1674-7968.2022.09.005

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Abstract Cajanus cajan is a medicinal and dietary woody legume with rich active substances and high economic value. Abiotic and biotic stresses, including drought and diseases, are important factors in the reduction of yield and quality of C. cajan; therefore, mining key C. cajan resistance genes and analyzing their functions are the focus of C. cajan breeding research. As an important member of the MYB (v-myb avian myeloblastosis viral) family of transcription factors, MYB-related (MYB-R) subfamily genes are thought to play an important role in adversity stress. In this study, 48 CcMYB-R (CcMYB-related) family genes (CcMYB-R1~CcMYB-R48) containing conserved structural domains of the MYB-R subfamily were screened by bioinformatic methods using Arabidopsis thaliana MYB-R subfamily genes for sequence alignment in the C. cajan genome. Analysis of transcriptome data revealed that CcMYB-R48 was significantly up-regulated and highly expressed under abscisic acid (ABA), Methyl Jasmonate (MeJA) stress treatments, suggested that it might be play an important function in stress response and hormone induction in C. cajan. Cloning and analysis of the CcMYB-R48 revealed that it had a typical MYB functional domain with high homology to Arabidopsis thaliana AT1G19000 and AT1G74840. The semi-quantitative RT-PCR (SqRT-PCR) revealed that CcMYB-R48 was significantly up-regulated in expression under both 6 and 12 h of drought stress, whereas no difference was observed under 6 and 12 h of high-temperature stress, suggested its possible involved in drought resistance in C. cajan. CcMYB-R48 overexpression vector was constructed and overexpressed CcMYB-R48 in C. cajan plants were established using the C. cajan instantaneous conversion system. The results showed that the overexpression plants had lower mortality of 19.44% under drought conditions, and their electrical conductivity, malondialdehyde (MDA) content and peroxidase (POD) content were significantly lower than those of the control plants, indicated that CcMYB-R48 could enhance the drought resistance of C. cajan. This study is important to further investigate the CcMYB-R subfamily genes of C. cajan and their drought resistance mechanism and to carry out molecular resistance breeding work.

Key words： Cajanus cajan MYB-related subfamily Transcriptome analysis Drought stress Functional verification

Received: 28 April 2022

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Corresponding Authors: *yang.qing1020@163.com

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	ZHANG Xiu-Qi
	DONG Bi-Ying
	SONG Zhi-Hua
	DU Ting-Ting
	LI Na
	XUE Jing-Yi
	CAO Hong-Yan
	WANG Tian-Yi
	SONG Yang-Bo
	MENG Dong
	YANG Qing

Cite this article:

ZHANG Xiu-Qi,DONG Bi-Ying,SONG Zhi-Hua, et al. Identification of MYB-related Subfamily Genes in Cajanus cajan and Functional Analysis of CcMYB-R48 Under Drought Stress[J]. 农业生物技术学报, 2022, 30(9): 1698-1712.

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http://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2022.09.005 OR http://journal05.magtech.org.cn/Jwk_ny/EN/Y2022/V30/I9/1698

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