Polymorphism of Growth Differentiation Factor 9 (GDF9) Gene and Its Correlation Analysis with Laying Performance in Jinding Duck (Anas platyrhynchos domestica)
QIN Yu-Ping1,2, CAO Yong-Qing2, LU Zhi-Xue1,2, ZHOU Wei3, LIN Yu-Mei4, LIU Guo-Fa3, LI Guo-Qin2, ZENG Tao2, LI Ang1, LU Li-Zhi2,*
1 College of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China; 2 Institute of Animal Husbandry and Veterinary Medicine, Zhejiang Academy of Agricultural Sciences, Hangzhou 310000, China; 3 Cherry Valley Agricultural Technology Co., Ltd., Zhoukou 461300, China; 4 Jingning Animal Husbandry and Veterinary Development Center, Jingning 323500, China
Abstract Growth differentiation factor 9 (GDF9), a growth factor secreted by oocytes, is involved in the regulation of ovarian development in mammals. In order to study the SNPs of GDF9 gene and find out the molecular markers related to laying performance of Jinding ducks (Anas platyrhynchos domestica), in this experiment, the direct sequencing method was used to detect GDF9 SNPs in 458 female Jinding ducks, and the correlations between different genotypes of each polymorphic site and the age at first egg as well as egg number at 300 d old were analyzed. The results showed that a total of 10 mutation sites were found in exon 1 and exon 2 of GDF9 of Jinding ducks. Among the mutation sites, 6 sites were missense mutation sites, and all sites had 3 genotypes; genetic analysis showed that 7 loci were moderately polymorphic (0.25<PIC<0.5), Hardy-Weinberg balance test showed that loci C344T and G557A were in equilibrium (P>0.05); Linkage disequilibrium analysis showed that there was no complete linkage disequilibrium among the 10 loci. Analysis on the correlation between polymorphic loci and laying performance indicated that sites C2111T and G2261T were significantly correlated with egg numbers at 300 d old (P<0.05), and sites T2345C and C2390T were significantly correlated with the age at first egg (P<0.05). In summary, these loci of GDF9 gene could be used as genetic markers for laying performance selection in Jinding ducks. The results may provide a reference for marker-assisted selection.
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