<em>RBP4</em>基因多态性与农华白鸭蛋用性状的关联性分析

doi:10.3969/j.issn.1674-7968.2021.08.013

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Abstract Egg production trait is one of the economically important traits that have long attracted attentions from duck (Anas platyrhynchos) breeders, and screening genetic markers associated with egg trait is of great significance for molecular marker-assisted breeding in duck. To explore the association of retinol-binding protein 4 (RBP4) gene polymorphisms with duck egg trait and identify the potential molecular markers, in this study, Nonghua White ducks were used as the experimental animals to detect single nucleotide polymorphisms (SNPs) in exons 1~6 and introns 1~3 of the RBP4 gene using PCR amplification combined with direct sequencing technologies, and their correlations with laying performance and egg quality traits were also analyzed. The results showed that one SNP namely g.266T>G was identified at the 266^th nucleotide within intron 1 of the RBP4 gene in Nonghua White duck, and the resulting 3 genotypes (TT, TG and GG) were present in this population, with 'T' being the dominant allele. Population genetics analysis showed that the heterozygosity (He), homozygosity (Ho), effective number of alleles (Ne) and polymorphic information content (PIC) at this mutation site was 0.408 7, 0.407 9, 1.697 7 and 0.277, respectively, being moderately polymorphic (0.25<P<0.50). Results from χ² test showed that this SNP site was not in Hardy-Weinberg equilibrium (P<0.05). Correlation analysis showed that genotypes at g.266T>G site were extremely significantly correlated with egg-white height (P<0.01), as manifested by higher egg-white height observed in individuals with TT genotype compared to those with TG and GG genotypes (P<0.01). Besides, this SNP could have the potential to influence egg production number, eggshell strength and egg weight, because individuals with GG genotype had higher egg production number and eggshell strength but lower egg weight compared to those with TG and TT genotypes. In summary, the g.266T>G SNP in intron 1 of the RBP4 gene has a significant impact on egg quality traits in Nonghua White duck and can be exploited as a candidate molecular marker for selection of duck egg production trait. This study provides a theoretical basis for future molecular marker-assisted breeding in Nonghua White duck.

Key words： Retinol-binding protein 4 (RBP4) SNP Nonghua White duck Laying performance Egg quality traits

Received: 24 November 2020

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Corresponding Authors: *wjw2886166@163.com; shenqiang.hu@sicau.edu.cn

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	Articles by authors
	LU Yao
	HU Shen-Qiang
	TANG Bin-Cheng
	OU-YANG Qing-Yuan
	LI Xue-Jian
	BAO De-Wei
	QING En-Hua
	LI Liang
	WANG Ji-Wen

Cite this article:

LU Yao,HU Shen-Qiang,TANG Bin-Cheng, et al. Association Analysis of RBP4 Gene Polymorphisms with Egg Traits in Nonghua White Duck (Anas platyrhynchos)[J]. 农业生物技术学报, 2021, 29(8): 1566-1573.

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http://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2021.08.013 OR http://journal05.magtech.org.cn/Jwk_ny/EN/Y2021/V29/I8/1566

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