金定鸭生长分化因子9 (<em>GDF9</em>)基因多态性及其与产蛋性能的相关分析

doi:10.3969/j.issn.1674-7968.2021.11.010

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Abstract Growth differentiation factor 9 (GDF9), a growth factor secreted by oocytes, is involved in the regulation of ovarian development in mammals. In order to study the SNPs of GDF9 gene and find out the molecular markers related to laying performance of Jinding ducks (Anas platyrhynchos domestica), in this experiment, the direct sequencing method was used to detect GDF9 SNPs in 458 female Jinding ducks, and the correlations between different genotypes of each polymorphic site and the age at first egg as well as egg number at 300 d old were analyzed. The results showed that a total of 10 mutation sites were found in exon 1 and exon 2 of GDF9 of Jinding ducks. Among the mutation sites, 6 sites were missense mutation sites, and all sites had 3 genotypes; genetic analysis showed that 7 loci were moderately polymorphic (0.25<PIC<0.5), Hardy-Weinberg balance test showed that loci C344T and G557A were in equilibrium (P>0.05); Linkage disequilibrium analysis showed that there was no complete linkage disequilibrium among the 10 loci. Analysis on the correlation between polymorphic loci and laying performance indicated that sites C2111T and G2261T were significantly correlated with egg numbers at 300 d old (P<0.05), and sites T2345C and C2390T were significantly correlated with the age at first egg (P<0.05). In summary, these loci of GDF9 gene could be used as genetic markers for laying performance selection in Jinding ducks. The results may provide a reference for marker-assisted selection.

Key words： Jinding duck Laying performance Growth differentiation factor 9 (GDF9) SNPs

Received: 12 January 2021

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Corresponding Authors: * lulizhibox@163.com

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	Articles by authors
	QIN Yu-Ping
	CAO Yong-Qing
	LU Zhi-Xue
	ZHOU Wei
	LIN Yu-Mei
	LIU Guo-Fa
	LI Guo-Qin
	ZENG Tao
	LI Ang
	LU Li-Zhi

Cite this article:

QIN Yu-Ping,CAO Yong-Qing,LU Zhi-Xue, et al. Polymorphism of Growth Differentiation Factor 9 (GDF9) Gene and Its Correlation Analysis with Laying Performance in Jinding Duck (Anas platyrhynchos domestica)[J]. 农业生物技术学报, 2021, 29(11): 2159-2165.

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http://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2021.11.010 OR http://journal05.magtech.org.cn/Jwk_ny/EN/Y2021/V29/I11/2159

[1] 高真真. 2014. 生长分化因子9及其信号通路蛋白(ALK5、Smad2和Smad4)在成年鹌鹑和鸡睾丸中的表达与定位[D]. 硕士学位论文, 山西农业大学, 导师: 贺俊平, pp. 20-34.
(Gao Z Z. 2014. Expression and localization of growth differ- entiation factor 9 and its signaling system proteins (ALK5, Smad2 and Smad4) in adult Japanese quail and chicken testes[D]. Thesis for M.S., Shanxi Agricultural University, Supervisor: He J P, pp. 20-34)
[2] 贾美婷, 李显耀, 康丽, 等. 2016. 寿光鸡GPR147基因5'调控区多态性与产蛋性状的关联分析[J]. 畜牧兽医学报, 47(01): 34-40.
(Jia M T, Li X Y, Kang L, et al. 2016. Correlation analysis between polymorphism of 5' regulatoryregion and egg production traits of Shouguangchicken GPR147 gene[J]. Acta Veterinaria et Zootech Nica Sinica, 47(01): 34-40.)
[3] 李碧侠, 储明星. 2002. 生长分化因子9基因的研究进展[J]. 中国畜牧兽医, 29(6): 33-36.
(Li B X, Chu M X. 2002. Advance on growth differentiation factor 9 gene[J]. China Animal Husbandry & Veterinary Medicine, 29(6): 33-36.)
[4] 任丽群. 2016. PRLR、GDF9和BMPR-IB基因多态性与宗地花猪繁殖性状的关联性分析[D]. 硕士学位论文, 贵州大学, 导师: 燕志宏, 刘若余, pp. 25-64.
(Ren L Q. 2016. Assoiciation of polymorphisms in PRLR、GDF9 and BMPR-IB gene with reproduction performance in zongdihua pig[D]. Thesis for M.S., Guizhou University, Supervisor: Yan Z H, Liu R Y, pp. 25-64.)
[5] 吴兆林, 李春苗, 黎寿丰, 等. 2020. GDF9 mRNA在不同品种鸡卵泡成熟过程中的表达规律研究[J].中国家禽, 42(10): 12-17.
(Wu Z L, Li C M, Li S F, et al. 2020. Expression regulation of GDF9 gene of different chicken breeds in follicle maturation[J]. China Poultry, 42(10): 12-17.)
[6] 王真, 王敏, 李铭, 等. 2020. 内蒙古白绒山羊GHR和GDF9基因多态性及其与生产性状的相关性分析[J].西北农林科技大学学报(自然科学版), 48(03): 1-8.
(Wang Z, Wang M, Li M, et al. 2020. Correlation between polymorphisms of GHR and GDF9 genes with production traits of Inner Mongolia cashmere goat[J]. Journal of Northwest A & F University (Natural Science Edition), 48(03): 1-8.)
[7] 王翔宇, 狄冉, 刘秋月, 等. 2020. 小鼠和绵羊BMP15和GDF9突变在卵泡发育中作用及其对排卵数的影响[J]. 农业生物技术学报, 28(10): 1870-1880.
(Wang X Y, Di R, Liu Q Y, et al. 2020. The effect of BMP15 and GDF9 gene mutations on follicular development and ovulation in mouse, 28(10): 1870-1880.)
[8] 赵茜, 努日比娅姆·麦麦提托合提, 宋玉坤, 等. 2020. GDF9对绵羊卵丘细胞增殖的影响[J].中国畜牧兽医, 47(10): 3289-3296.
(Zhao Q, Maimaititouheti N, Song Y K, et al. 2020. Effect of GDF9 on sheep cumulus cell proliferation[J]. China Animal Husbandry & Veterinary Medicine, 47(10): 3289-3296.)
[9] Amirpour N H, Khansefid M, Mahmoud G G, et al.2020. Identification of polymorphisms in the oocyte-derived growth differentiation growth factor 9 (GDF9) gene associated with litter-size in New Zealand sheep (Ovis aries) breeds[J]. Reproduction in Domestic Animals, 55(11): 1585-1591.
[10] Bi Y, Li J, Wang X, et al.2020. Two novel rare strongly linked missense SNPs (P27R and A85G) within the GDF9 gene were significantly associated with litter size in Shanbei White Cashmere (SBWC) Goats[J]. Frontiers in Veterinary Science, 7(7): 406-406.
[11] Cepica S, Procházka R, Civánová K, et al.2004. Partial molecular characterization and mapping of the GDF9 gene to porcine chromosome 2[J]. Animal Genetics, 35(3): 261-262.
[12] Chang H, Brown C W, Matzuk M M.2002. Genetic analysis of the mammalian transforming growth factor-β superfamily[J]. Endocrine Reviews, 23(6): 787-823.
[13] Fitzpatrick S L, Sindoni D M, Shughrue P J, et al.1998. Expression of growth differentiation factor-9 messenger ribonucleic acid in ovarian and nonovarian rodent and human tissues 1[J]. Endocrinology, 139(5): 2571-2578.
[14] Hanrahan J P, Gregan S M, Mulsant P, et al.2004. Mutations in the genes for oocyte-derived growth factors GDF9 and BMP15 are associated with both increased ovulation rate and sterility in Cambridge and Belclare sheep (Ovis aries)[J]. Biology of Reproduction, 70(4): 900-909.
[15] Huang H Y, Liang Z, Li S F, et al.2015. Polymorphism identification in BMP15 and GDF9 genes and their association with egg production in chickens[J]. British Poultry Science, 56(3): 277-83.
[16] Lee K A, Volentine K K, Bahr J M.1998. Two steroidogenic pathways present in the chicken ovary: Theca layer prefers delta 5 pathway and granulosa layer prefers delta 4 pathway[J]. Domestic Animal Endocrinology, 15(1): 1-8.
[17] Liu L, Cui Z, Xiao Q, et al.2018. Polymorphisms in the chicken growth differentiation factor 9 gene associated with reproductive traits[J]. Biomed Research International, 2018: 9345473.
(https://doi.org/10.1155/2018/9345473)
[18] Lou Q, Li T, Wu P, et al.2019. Polymorphism identification in GDF9 gene and its association analysis with reproduction traits in Jinghai Yellow chicken[J]. Animal Biotechnology, 30(4): 332-341.
[19] McGrath S A, Esquela A F, Lee S J.1995. Oocyte-specific expression of growth/differentiation factor-9[J]. Molecular Endocrinology, 9(1): 131-136.
[20] Mcnatty K P, Smith P, Moore L G, et al.2005. Oocyte expressed genes affecting ovulation rate[J]. Molecular and Cellular Endocrinology, 234(1): 57-66.
[21] McPherron A C, Lee S J.1993. GDF-3 and GDF-9: Two new members of the transforming growth factor-beta superfamily containing a novel pattern of cysteines[J]. Biological Chemistry, 268(5): 3444-3449.
[22] Nicholls P K, Harrison C A, Gilchrist R B, et al.2009. Growth differentiation factor 9 is a germ cell regulator of Sertoli cell function[J]. Endocrinology, 150(5): 2481-2490.
[23] Palmer J S, Zhao Z Z, Hoekstra C, et al.2006. Novel variants in growth differentiation factor 9 in mothers of dizygotic twins[J]. The Journal of Clinical Endocrinology and Metabolism, 91(11): 4713-4716.
[24] Qin N, Liu Q, Zhang Y Y, et al.2015. Association of novel polymorphisms of forkhead box L2 and growth differentiation factor-9 genes with egg production traits in local Chinese Dagu hens[J]. Poultry Science, 94(1): 88-95.
[25] Sadighi M, Bodensteiner K J, Beattie A E, et al.2002. Mapping of ovine growth differentiation factor-9 (GDF9) to sheep chromosome 5[J]. Animal Genetics, 33(3): 244-245.
[26] Wang Y, Nicholls P K, Stanton P G, et al.2009. Extra-ovarian expression and activity of growth differentiation factor 9[J]. The Journal of Endocrinology, 202(3): 419-430.
[27] Wang Y, Xu H Y, Gilbert E R, et al.2014. Detection of SNPs in the TBC1D1 gene and their association with carcass traits in chicken[J]. Gene, 547(2): 288-294.