红花脂肪酸脱氢酶基因(<em>CtFAD2</em>-<em>1</em>)启动子克隆及功能分析

doi:10.3969/j.issn.1674-7968.2021.04.005

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Abstract Linoleic acid is main polyunsaturated fatty acids in plant lipids, which plays key roles in plant metabolism. Fatty desaturase 2 (FAD2) is a key enzyme for linoleic acid biosynthesis, CtFAD2-1 gene has the highest activity and was mainly responsible for the biosynthesis of linoleic acid in safflower (Carthamus tinctorius) seed. In this study, an 821 bp promoter region of CtFAD2-1 gene from safflower was cloned. Four 5'-deletion constructs of 821 bp (P1), 564 bp (P2), 230 bp (P3), 60 bp (P4) were constructed and functionally characterized in Arabidopsis thaliana by using transgenic approach. Computational analysis found that the abiotic stress responsive cis-elements like heat responsive element (HSE), low temperature response (LTR), MYB response (MBS) etc, as well as wound, fungus, methyljasmonate responsive motifs existed in the promoter. The histochemical staining of GUS revealed that the flower, mature seed and silique wall were stained. In addition, the transgenic plants treated by drought, salt, cold, high-temperature, wound and ABA hormone as well as control plants were also stained. The -230~-60 bp might be the key region for GUS activity in A. thaliana tissues. CtFAD2-1 gene was not seed-specific and it could be induced by an assortment of abiotic stresses. This research plays an indispensable role in furthering the research related to the regulation of CtFAD2-1 gene.

Key words： Safflower Fatty acid desaturase (FAD) Functional analysis Promoter

Received: 13 July 2020 Published: 01 April 2021

ZTFLH:

S567.9

Corresponding Authors: * ewuwei@gmail.com

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	LI Dan-Dan
	WANG Qing
	XU Xin
	YU Jing-Sheng
	WU Wei

Cite this article:

LI Dan-Dan,WANG Qing,XU Xin, et al. Cloning and Functional Analysis of Fatty Desaturase Gene (FAD2-1) Promoter from Safflower (Carthamus tinctorious)[J]. 农业生物技术学报, 2021, 29(4): 663-672.

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http://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2021.04.005 OR http://journal05.magtech.org.cn/Jwk_ny/EN/Y2021/V29/I4/663

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