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| Construction of SLAF Genetic Linkage Map and Mapping of Major QTL for Peduncle Length in Melon (Cucumis melo) |
| DAI Dong-Yang*, FAN Lei*, ZENG Shuang, WANG Ling, SHENG Yun-Yan**, LI Zheng-Zhou, ZHANG Fan |
| College of Horticulture and Landscape Architecture, Heilongjiang Bayi Agricultural Reclamation University, Daqing 163319, China |
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Abstract Long peduncle varieties of melon (Cucumis melo) are beneficial to large-scale production and mechanized harvesting. In present study, long peduncle melon '1244' and short peduncle melon 'MS-5' were used to make crossing and constructed F2 segregation population, using specific locus amplified fragment sequencing sequencing (SLAF-seq) technology to construct melon genetic map, combing with SSR molecular markers, to detect fruit peduncle length (fpl) QTL locus. The results showed, a genetic map of 12 linked melon populations, with 10 595 markers were constructed, the total distance was 1 383.88 cM and the average distance between the markers was 0.13 cM; melon peduncle length was controlled by 2 pairs of main genes, fpl1.1 was located on chromosome 1, the phenotypic contribution rate was 12.2%, QTL candidate interval was between 57 524 and 270 836 in melon scaffold00060, located between CMSSR05887 and CMSSR05892; fpl8.1 was located on chromosome 8, the phenotypic contribution rate was 13.2%, QTL candidate interval was between 94 748 and 105 498 in melon scaffold00058, located between CMSSR20549 and CMSSR20495. The results provide a theoretical basis for the study of melon molecular marker assisted selection breeding and melon mechanized harvesting.
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Received: 22 June 2020
Published: 01 April 2021
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Corresponding Authors:
** shengyunyan12345@163.com
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| About author:: * The authors who contribute equally |
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