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| Identification and Genetic Diversity Analysis of Litchi chinensis Colletotrichum spp. in Hainan |
| LI Shao-Ka, ZHAO Ya, WANG Xiang-He, HU Fu-Chu, CHEN Zhe, FAN Hong-Yan* |
| Tropical Fruit Trees Institute, Hainan Academy of Agricultural Sciences/Investigation Station of Tropical Fruit Trees, Ministry of Agriculture/Key Laboratory of Tropical Fruit Tree Biology of Hainan Province/Research Center of Tropical Fruit Tree Breeding Engineering Technology of Hainan Province, Haikou 571100, China |
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Abstract Anthrax is an important disease in litchi (Litchi chinensis) production, which severely limits the development of the litchi industry. In order to clarify the species composition, the dominant population of the pathogen of litchi anthracnose in Hainan, and the correlativity between the distribution of different populations in the spatial area of Hainan and the geographical source. This article conducted an investigation and sampling of the occurrence of anthracnose tissues in the litchi planting areas of Hainan from 2018 to 2019, and the isolation and purification of pathogenic microorganisms and the verification of Koch's law was proceeded; Using combined analysis of multiple genes (internal transcribed spacers (ITS), partial actin (ACT), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), chitin synthetase (CHS-1), β-tubulin (TUB), giutamine synthetase (GS) and mating type (ApMat)) to research the species composition, distribution and geographical relationship of Hainan litchi anthracnose, as well as the genetic diversity of populations in different regions. A total of 79 strains of litchi anthracnose pathogens were isolated in this study. The results showed that the mainly species of pathogens causing litchi anthracnose were C. acutadum and the complex group C. gloeospoiorides species, including C. fructicola, C. siamense, C. asianum, C. musae, C. kahawas subsp. kahawae and C. horri, which had greater abundance in species composition in Hainan, as the dominant pathogenic group was C. gloeosporioides; Various strains do not have geographic regionality in origin. The geographical regionality and the genetic distance of each geographical strain were relatively close, and the distribution of pathogens had no obvious correlation with geography; the source of genetic variation was mainly among geographic populations. This study provides a theoretical basis for a more in-depth study of the occurrence rules, control measures of litchi anthracnose and further breeding for disease resistance.
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Received: 15 June 2020
Published: 01 April 2021
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Corresponding Authors:
* fanhongyan_1979@126.com
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