牦牛<em>CAV1</em>基因克隆及其在雌性生殖系统主要器官中的表达定位

doi:10.3969/j.issn.1674-7968.2020.04.010

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Abstract Caveolin-1 (Caveolin-1, CAV1) is a membrane protein associated with endocytosis, extracellular matrix organization, cholesterol distribution, cell migration and signaling.The aim of the present study was to explore the expression and biological function of CAV1 in the reproductive system of female yak (Bos grunniens) under normal physiological conditions. In this study, the uterus, ovaries and fallopian tubes of female yaks at different reproductive stages (follicula, luteal and gestation) were used as materials. The mRNA and protein expression of CAV1 were analyzed by gene cloning, qRT-PCR, Western blot and immunohistochemistry (IHC). The results showed that the CAV1 gene (GenBank No. MN127963) was highly conserved. The CAVI gene sequence of yak was different from ordinary cattle (Bos taurus), but the encoded amino acid was constant. The CAVI gene of yak had the closest relatives to Bos taurus and Ovis aries, and the farthest relatives to Felis catus and Equus saddle. and the encoded protein was a stable hydrophobic membrane protein. CAV1 was expressed in the main organs of the female yak reproductive system, while the expression level of CAV1 protein in the oviduct, ovary and uterus in the follicular phase was significantly higher than that in the luteal phase and gestational period (P<0.05). The expression level of CAV1 protein in the fallopian tube of the gestational period was significantly higher than that in the luteal phase (P<0.05). The expression level of CAV1 protein in the ovary and uterus of the luteal phase was significantly higher than that in the gestation phase(P<0.05). Immunohistochemistry results showed that CAV1 is expressed in the oviduct mucosa epithelium, serous gland, ovarian follicle membrane, follicular granule layer, luteal cells, reproductive epithelium, endometrium and uterine gland. This study provides a theoretical basis for further exploration of CAV1 involved in mammalian reproductive physiology regulation, which is helpful to the study of the breeding rules of mammals in the plateau.

Key words： Yak Caveolin-1(CAV1) Endocytosis

Received: 25 September 2019

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Corresponding Authors: * sjyu@163.com

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	ZHANG Rui
	WANG Jing-Lei
	PAN Yang-Yang
	WANG Jing-Yu
	HU Xue-Quan
	MA Rui
	CUI Yan
	YU Si-Jiu

Cite this article:

ZHANG Rui,WANG Jing-Lei,PAN Yang-Yang, et al. Cloning of Yak (Bos grunniens) CAV1 Gene and Its Expression in Major Organs of Female Reproductive System[J]. 农业生物技术学报, 2020, 28(4): 681-692.

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http://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2020.04.010 OR http://journal05.magtech.org.cn/Jwk_ny/EN/Y2020/V28/I4/681

[1] 黄堃. 2018. CAV1和JAK在原始卵泡形成中的功能与机制研究[D]. 博士学位论文, 中国农业大学, 导师: 夏国良. pp. 16-18.
(Huang K.2018. Functions and mechanisms of CAV1 and JAK in primordial follicle formation[D]. Thesis for Ph. D., China Agricultural University, Supervisor: Xia G L, pp. 16-18.)
[2] 官久强, 张海波, 罗晓林. 2019. 舍饲牦牛与犏牛生长性能、屠宰性能、肉品质和肌内脂肪沉积相关基因表达的比较分析[J]. 动物营养学报, 31(06): 2659-2665.
(Guan J Q, Zhang H B, Luo X L.2019. Comparative study on growth performance, slaughter performance, meat quality and intramuscular fat deposition related gene expression between dzo and yak in house feeding[J]. Chinese Journal of animal nutrition, 31(06): 2659-2665.)
[3] 刘玉花. 2019. Caveolin-1对2型糖尿病小鼠内皮祖细胞功能的影响研究[D]. 硕士学位论文, 广西医科大学, 导师: 戴霞. pp. 10.
(Liu Y H.2019. Effect of caveolin-1 on functions of endothelial progenitor cells in mice with type 2 diabtes[D]. Thesis for M.S., Guangxi Medical University. Supervisor: Dai X. pp. 10.)
[4] 沈霞芬. 2011. 家畜组织学与胚胎学[M]. 北京: 中国农业出版社, pp: 200-201.
(Shen X F.2011. Livestock Histolo-gy and Embryology[M]. Beijing: China Agricultural Press, pp: 200-201.)
[5] 王靖雷, 王萌, 潘阳阳, 等. 2019. Artemin在牦牛主要生殖器官和孤雌激活胚胎中的表达及定位[J]. 农业生物技术学报, 27(3): 431-440.
(Wang J L, Wang M, Pan Y Y, et al.2019. Artemin expression and localization in main reproductive organs and parthenogenetic embryos of yak (Bos grunniens)[J]. Journal of Agricultural Biotechnology, 27(3): 431-440)
[6] 赵兴绪. 2011. 兽医产科学[M]. 北京:中国农业出版社, pp. 86.
(Zhao X X.2011. Veterinary Obstetrics [M]. Beijing: China Agricultural Press, pp. 86.)
[7] Bianco C, Strizzi L, Mancino M, et al.2008. Regulation of cripto-1 signaling and biological activity by caveolin-1 in mammary epithelial cells[J]. American Journal of Pathology, 172(2): 345-357.
[8] Bullejos M, Bowles J, Koopman P.2002. Extensive vascularization of developing mouse ovaries revealed by caveolin-1 expression[J]. Developmental Dynamics, 225: 95-99.
[9] Campos A, Burgos-Ravanal R, González M F, et al.2019. Cell intrinsic and extrinsic mechanisms of caveolin-1 enhanced metastasis[J]. Biomolecules, 9(8), Doi: 10.3390/biom9080314.
[10] Castillo Bennett J, Silva P, Martinez, et al.2018. Hypoxia-induced caveolin-1 expression promotes migration and invasion of tumor cells[J]. Current molecular medicine, 18(4): 199-206.
[11] Chen Z, Cao J, Zhou J.2014. Interaction of caveolin-1 with ATG12-ATG5 system suppresses autophagy in lung epithelial cells[J]. American Journal of Physiology Lung Cellular and Molecular Physiology, 306(11): 1016-1025.
[12] Engelman J A, Chu C, Lin A, et al.1998. Caveolin-mediated regulation of signaling along the p42/44 MAP kinase cascade in vivo[J]. FEBS Letters, 428(3): 205-211.
[13] Francisca M Real, F. David Carmona, et al.2012. Pattern and density of vascularization in mammalian testes, ovaries, and ovotestes[J]. Journal of Experimental Zoology Part B Molecular and Developmental Evolution, 318(3): 170-181.
[14] Geletu M, Mohan R, Arulanandam R, et al.2018. Reciprocal regulation of the Cadherin-11/Stat3 axis by caveolin-1 in mouse fibroblasts and lung carcinoma cells[J]. Biochimica et Biophysica Acta-Molecular Cell Research, 1865(5): 794.
[15] Hiyama T, Tanaka S, Shima H, et al.2003. Somatic mutaion of mitochondrial DNA in Helicobacter pylori-associated chronic gastritis in patients with and without gastric cancer[J]. International Journal of Molecular Medicine, 12(2): 169-174.
[16] Huang K, Dang Y, Zhang P, et al.2018. CAV1 regulates primordial follicle formation via the Notch2 signalling pathway and is associated with premature ovarian insufficiency in humans[J]. Human Reproduction, 33: 2087-2095.
[17] Li H Z, Sun X, Stavreusevers A, et al.2004. Effect of mifepristone on the expression of cytokines in the human Fallopian tube[J]. Molecular Human Reproduction, 10(7): 489.
[18] Nah J, Yoo S M, Jung S, et al.2017. Phosphorylated CAV1 activates autophagy through an interaction with BECN1 under oxidative stress[J]. Cell Death and Disease, 8(5): e2822.
[19] Nohe A.2005. Dynamics and interaction of caveolin-1 isoforms with BMP-receptors[J]. Journal of Cell Science, 118(3): 643-650.
[20] Nwosu Z C, Ebert M P, Dooley S, et al.2016. Caveolin-1 in the regulation of cell metabolism: A cancer perspective[J]. Molecular Cancer, 15(1): 71.
[21] Okamoto T, Schlegel A, Scherer P E, et al.1998. Caveolins, a family of scaffolding proteins for organizing \"preassembled signaling complexes\" at the plasma membrane[J]. Journal of Biological Chemistry, 273(10): 5419-5422.
[22] Parzych K R, Klionsky D J.2014. An overview of autophagy: Morphology, mechanism, and regulation[J]. Antioxidants & Redox Signaling, 20(3): 460-473.
[23] Rahman M, Sadygov R G.2017. Predicting the protein half-life in tissue from its cellular properties[J]. PLoS ONE, 12(7): e0180428.
[24] Razani B, WoodmanS E, LisantiM P.2002. Caveolae: From cell biology to animal physiology[J]. Pharmacological Reviews, 54: 431-467.
[25] Satterfield M C, Hayashi K, Song G, et al.2008. Progesterone regulates FGF10, MET, IGFBP1, and IGFBP3 in the endometrium of the Ovine Uterus[J]. Biology of Reproduction, 79(6): 1226-1236.
[26] Scherer P E, Lewis R Y, Volonte D, et al.1997. Cell-type and tissue-specific expression of Caveolin-2: Caveolins 1 and 2 co-localize and form a stable hetero-oligomeric complex in vivo[J]. Journal of Biological Chemistry, 272(46): 29337-29346.
[27] Schlegel A.2000. A molecular dissection of caveolin-1 membrane attachment and oligomerization. Two separate regions of the caveolin-1 c-terminal domain mediate membrane binding and oligomer/oligomer interactions in vivo[J]. Journal of Biological Chemistry, 275(28): 21605-21617.
[28] Shi Y, Tan S H, Ng S, et al.2015. Critical role of CAV1/caveolin-1 in cell stress responses in human breast cancer cells via modulation of lysosomal function and autophagy[J]. Autophagy, 11(5): 769-784.
[29] Sohn J, Brick R M, Tuan R S.2016. From embryonic development to human diseases: The functional role of caveolae/caveolin[J]. Birth Defects Research Part C: Embryo Today: Reviews, 108(1): 45-64.
[30] Tiwari A, Copeland C A, Han B, et al.2016. Caveolin-1 is an aggresome-inducing protein[J]. Scientific Reports, 6(1): 38681.
[31] Trimmer C, Bonuccelli G, Katiyar S, et al.2013. Cav1 suppresses tumor growth and metastasis in a murine model of cutaneous scc through modulation of MAPK/AP-1 activation[J]. The American Journal of Pathology, 182(3): 992-1004.
[32] Vanorny D A, Prasasya R D, Chalpe A J, et al.2014. Notch Signaling regulates ovarian follicle formation and coordinates follicular growth[J]. Molecular Endocrinology, 28(4): 499-511.
[33] Wang Z, Wang N, Liu P, et al.2015. Caveolin-1, a stress-related oncotarget, in drug resistance[J]. Oncotarget, 6(35): 37135-37150.
[34] Williams T M, Lisanti M P.2004. The caveolin proteins[J]. Genome Biology, 5(3): 214.
[35] Wu J, Di D, Zhao C, et al.2018. Clinical significance of gli-1 and caveolin-1 expression in the human small cell lung cancer[J]. Asian Pacific Journal of Cancer Prevention: APJCP, 19(2): 401.
[36] Xu J, Gridley T.2013. Notch2 is required in somatic cells for breakdown of ovarian germ-cell nests and formation of primordial follicles[J]. BMC Biology, 11(1): 13.
[37] Yamao T, Yamashita Y I, Yamamura K, et al.2019. Cellular senescence, represented by expression of caveolin-1, in cancer-associated fibroblasts promotes tumor invasion in pancreatic cancer[J]. Annals of Surgical Oncology, 26: 1552-1559.
[38] Yan W, Yongxi S, Xiaofang C, et al.2018. Caveolin-1 enhances RANKL induced gastric cancer cell migration[J]. Oncology Reports, 40, 1287-1296.
[39] Yu S J, Huang Y M, Chen B X.1993. Reproductive patterns of the yak. I. Reproductive phenomena of the female yak[J]. British Veterinary Journal, 149(6): 579-583.
[40] Zhao L, Zhou S, Zou L, et al.2013. The expression and functionality of stromal caveolin 1 in human adenomyosis[J]. Human Reproduction, 28(5): 1324-1338.
[41] Zheng K, Trivedi M, Siahaan T.2006. Structure and function of the intercellular junctions: Barrier of paracellular drug delivery[J]. Current Pharmaceutical Design, 12(22): 2813-2824.