Expression and Localization of LHR and PRLR Genes in Testicles of Yak (Bos grunniens) at Different Developmental Stages
MA Fu-Long1,2, CHU Min1, CHANG Yong-Fang1,2, BAO Peng-Jia1, GUO Xian1, Qudratullah Kalwar1, MA Qi-Cai1,2, PAN He-Ping2, YAN Ping1*
1 Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Aicultural Sciences/ Gansu Key Laboratory for Yak Breeding Engineering of Gansu Province, Lanzhou 730050, China; 2 College of Life Science and Engineering, Northwest University for Nationlities, Lanzhou 730030, China
Abstract Luteinizing hormone receptor (LHR) and prolactin receptor (PRLR) genes play essential roles in mammalian reproductive development. But still no any available research regarding these genes in yak (Bos grunniens). Therefore this study was planned to explore the role of LHR and PRLR genes in different tissues of Yak during different developmental ages (6, 24, 36 and 72 months). The mRNA expression levels of LHR and PRLR in the testis tissues of yak at different developmental stages and organs of yak were detected by qRT-PCR, while the protein expression of LHR and PRLR were evaluated by immunohistochemistry.The results showed that mRNA expression levels of LHR and PRLR genes in yak testis increased significantly from infancy to sexual maturity (P<0.01), but there was no significant difference between adult and sexual maturity. The expression level of LHR gene mRNA in different organs of adult yak was higher in testis than in kidney, skeletal muscle and lung (P<0.05). While the expression level of PRLR mRNA was higher in kidney, followed by skeletal muscle, testis and spleen (P<0.05). Additionally, immunohistochemistry findings revealed that LHR and PRLR proteins were located in testicular supporting cells, mesenchymal cells, primary spermatocytes and secondary spermatocytes, and they were increased gradually from infancy to sexual maturity stage and tended to be stable in adulthood. These findings displayed that LHR and PRLR may be vital for sperm maturation, in the biological functions of interstitial cells, and in regulating the development of other non-gonadal tissues. These results provide an important theoretical basis for revealing the molecular mechanism of testis development and reproductive regulation in yaks.
MA Fu-Long,CHU Min,CHANG Yong-Fang, et al. Expression and Localization of LHR and PRLR Genes in Testicles of Yak (Bos grunniens) at Different Developmental Stages[J]. 农业生物技术学报, 2020, 28(4): 672-680.
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