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| Cloning, Subcellular Localization and Expression Analysis of MAPK Genes from Vitis yeshanesis |
| ZHU Zi-Guo1, ZHANG Qing-Tian1, LI Xiu-Jie1, HAN Zhen1, LI Gui-Rong2, LI Bo1, * |
1 Shandong Institute of Pomology, Taian 271000, China;
2 College of Horticulture and Landscape Architecture, Henan Institute of Science and Technology, Xinxiang 453003, China |
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Abstract Mitogen-activated protein kinase (MAPK) cascade pathway plays an important role in plant growth and development and signal transduction in response to stress. However less the role of MAPK family members were reported in Chinese wild grape (Vitis sp.). In this study, two novel MAPK genes VyMAPK2 and VyMAPK3 (GenBank No. MK942078, MK942077) were obtained from Vitis yeshanesis. VyMAPK2 and VyMAPK3 proteins belonged to the B and A subgroup of the MAPK family, respectively, which all contained 11 conserved protein kinase subdomains, TEY motifs, activation-loop, common docking domain, P-loop and C-loop. Subcellular localization analysis showed that VyMAPK2 was mainly distributed in the nucleus, VyMAPK3 was mainly distributed in the nucleus and cytoplasm. Gene expression analysis showed that VyMAPK2 was mainly expressed in root and VyMAPK3 was mainly expressed in leaves. Under exogenous hormone treatments, auxin, gibberellin, 6-benzyladenine, ethylene, abscisic acid, salicylic acid, and methyl jasmonate could significantly induced the expression of VyMAPK3, but there was no significant change of the expression of VyMAPK2 (P<0.05). Under stress, drought, high salinity and low temperature could significantly induced the expression of VyMAPK3 gene (P<0.05), while VyMAPK2 was only induced by drought. The above results showed that VyMAPK3 gene played an important role in the development and resistance to external stresses in V. yeshanesis. This study will be helpful to understand the stress resistance mechanism of wild grape in China and supply the genes for breeding.
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Received: 21 June 2019
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Corresponding Authors:
* sdtalibo@163.com
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1 陈景新. 1979. “燕山葡萄”—一种兼备高糖与高抗性的野生资源. 中国果树, (1): 11-15.
(Chen J X. 1979. Vitis yeshanesis—a wild resource with high sugar content and high resistance[J]. China Fruits, (1): 11-15.)
2 林金辉, 党峰峰, 陈建鸿, 等. 2017. CaMAPK9过表达可显著提高拟南芥耐盐水平. 农业生物技术学报, 25(10): 1612-1621.
(Lin J H, Dang F F, Chen J H, et al.2017. Overexpression of CaMAPK9 significantlly enhanced tolerance to salt stress in Arabidopsis thaliana[J]. Journal of Agricultural Biotechnology, 25(10): 1612-1621.).
3 夏思哲, 李铁梅, 李凤菊, 等. 2017. 野生葡萄‘燕山-1’ב河岸-3’种间杂交F1代植株耐盐性鉴定[J]. 西北林学院学报, 32(1): 150-156.
(Xia S Z, Li T M, Li F J, et al.2017. Identification of salt tolerance of in vitro F1 hybrid progenies of Yanshan-1×Hean-3[J]. Journal of Northwest Forestry University, 32(1): 150-156.)
4 张今今, 王跃进, 王西平, 等. 2003. 葡萄总RNA提取方法的研究[J]. 果树学报, 20(3): 178-181.
(Zhang J J, Wang Y J, Wang X P, et al.2003. An improved method for rapidly extracting total RNA from Vitis[J]. Journal of Fruit Science, 20(3): 178-181.).
5 Brodersen P, Petersen M, Bjorn Nielsen H, et al.2006. Arabidopsis MAP kinase 4 regulates salicylic acid- and jasmonic acid/ethylene-dependent responses via EDS1 and PAD4[J]. The Plant Journal, 47: 532-546.
6 Brunet A, Roux D, Lenormand P, et al.1999. Nuclear translocation of p42/p44 mitogen-activated protein kinase is required for growth factor-induced gene expression and cell cycle entry[J]. The EMBO Journal, 18: 664-674.
7 Gao M, Liu J, Bi D, Zhang Z, et al.2008. MEKK1, MKK1/MKK2 and MPK4 function together in a mitogen-activated protein kinase cascade to regulate innate immunity in plants[J]. Cell Research, 18: 1190-1198.
8 Group M.2002. Mitogen-activated protein kinase cascades in plants: A new nomenclature[J]. Trends in Plant Science, 7: 301-308.
9 Hamel L P, Nicole M C, Sritubtim S, et al.2006. Ancient signals: Comparative genomics of plant MAPK and MAPKK gene families[J]. Trends in Plant Science, 11:192-198.
10 Hyun T K, Kim J S, Kwon S Y, et al.2010. Comparative genomic analysis of mitogen activated protein kinase gene family in grapevine[J]. Genes & Genomics, 32: 275-281.
11 Jaillon O, Aury J M, Noel B, et al.2007. The grapevine genome sequence suggests ancestral hexaploidization in major angiosperm phyla[J]. Nature, 449: 463-467.
12 Jonak C, Okresz L, Bogre L, et al.2002. Complexity, cross talk and integration of plant MAP kinase signalling[J]. Current Opinion in Plant Biology, 5: 415-424.
13 Kim H S, Park S C, Ji C Y, et al.2016. Molecular characterization of biotic and abiotic stress-responsive MAP kinase genes, IbMPK3 and IbMPK6, in sweetpotato[J]. Plant Physiology and Biochemistry, 108: 37-48.
14 Kovtun Y, Chiu W L, Tena G, et al.2000. Functional analysis of oxidative stress-activated mitogen-activated protein kinase cascade in plants[J]. Proceedings of the National Academy of Sciences of the USA, 97: 2940-2945.
15 Kumar K R, Srinivasan T, Kirti P B.2009. A mitogen-activated protein kinase gene, AhMPK3 of peanut: Molecular cloning, genomic organization, and heterologous expression conferring resistance against Spodoptera litura in tobacco[J]. Molecular Genetics and Genomics, 282: 65-81.
16 Lee J S, Wang S, Sritubtim S, et al.2009. Arabidopsis mitogen-activated protein kinase MPK12 interacts with the MAPK phosphatase IBR5 and regulates auxin signaling[J]. The Plant Journal, 57: 975-985.
17 Liu J Z, Horstman H D, Braun E, et al.2011. Soybean homologs of MPK4 negatively regulate defense responses and positively regulate growth and development[J]. Plant Physiology, 157: 1363-1378.
18 Livak K J, Schmittgen T D.2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2-Delta Delta CT. Method[J]. Methods, 25: 402-408.
19 Mockaitis K, Howell S H.2000. Auxin induces mitogenic activated protein kinase MAPK activation in roots of Arabidopsis seedlings[J]. The Plant Journal , 24: 785-796.
20 Musielak T J, Bayer M.2014. YODA signalling in the early Arabidopsis embryo[J]. Biochemical Society Transactions, 42: 408-412.
21 Nicole M C, Hamel L P, Morency M J, et al.2006. MAP-ping genomic organization and organ-specific expression profiles of poplar MAP kinases and MAP kinase kinases[J]. BMC Genomics, 7:223.
22 Ouaked F, Rozhon W, Lecourieux D, et al.2003. A MAPK pathway mediates ethylene signaling in plants[J]. The EMBO Journal, 22: 1282-1288.
23 Petersen M, Brodersen P, Naested H, et al.2000. Arabidopsis map kinase 4 negatively regulates systemic acquired resistance[J]. Cell, 103: 1111-1120.
24 Ren D, Liu Y, Yang K Y, et al.2008. A fungal-responsive MAPK cascade regulates phytoalexin biosynthesis in Arabidopsis[J]. Proceedings of the National Academy of Sciences of the USA, 105: 5638-5643.
25 Singh P, Mohanta T K, Sinha A K.2015. Unraveling the intricate nexus of molecular mechanisms governing rice root development: OsMPK3/6 and auxin-cytokinin interplay[J]. PLOS ONE, 10: e0123620.
26 Tena G, Asai T, Chiu W L, etal.2001. Plant mitogen-activated protein kinase signaling cascades[J]. Current opinion in Plant Biology, 4: 392-400.
27 Teige M, Scheikl E, Eulgem T, et al.2004. The MKK2 pathway mediates cold and salt stress signaling in Arabidopsis[J]. Molecular Cell, 15: 141-152.
28 Wang L, Liu Y, Cai G, et al.2014. Ectopic expression of ZmSIMK1 leads to improved drought tolerance and activation of systematic acquired resistance in transgenic tobacco[J]. Journal of Biotechnology, 172: 18-29.
29 Xiong L, Yang Y.2003. Disease resistance and abiotic stress tolerance in rice are inversely modulated by an abscisic acid-inducible mitogen-activated protein kinase[J]. The Plant Cell, 15: 745-759.
30 Yang Y, He M, Zhu Z, et al.2012. Identification of the dehydrin gene family from grapevine species and analysis of their responsiveness to various forms of abiotic and biotic stress[J]. BMC Plant Biology, 12: 140.
31 Yuan B, Shen X, Li X, et al.2007. Mitogen-activated protein kinase OsMPK6 negatively regulates rice disease resistance to bacterial pathogens[J]. Planta, 226: 953-960.
32 Zhou Y, Zhang D, Pan J, et al.2012. Overexpression of a multiple stress-responsive gene, ZmMPK4, enhances tolerance to low temperature in transgenic tobacco[J]. Plant Physiology and Biochemistry, 58: 174-181. |
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